Impact of lymphocyte culture media on the number of metaphases and chromosome band resolution
Journal Title: Advances in Hygiene and Experimental Medicine - Year 2016, Vol 70, Issue
Abstract
A special type of differential staining of chromosomes is replication banding. This staining technique reveals the band pattern characteristic of each homologous pair of chromosomes, which is a reflection of heterogeneous euchromatin structure. Banding enables identification of homologous chromosomes and detection of chromosomal aberrations, both structural and numerical. Slide preparation requires knowledge of many techniques, and the procedure is often different for each laboratory. The aim of the study was to determine the effect of selected media for lymphocyte cultures on the number of metaphases and the band resolution of chromosomes. The study was carried out using cell cultures from whole peripheral blood. The slides were stained by the GTG method. After their removal from the water bath they were immersed in trypsin solution, then rinsed in PBS solution and stained in Giemsa solution. After staining they were rinsed again and left to dry. The study confirmed the effect of selected commercially available cell media on the number of metaphases and band resolution of chromosomes, which have not previously been described. In all of the tests performed, the cell culture, fixation, slide preparation (automatic method), staining, and number of reagents were identical.
Authors and Affiliations
Piotr Chomik, Magdalena Gryzinska, Justyna Batkowska, Grazyna Jezewska-Witkowska, Janusz Kocki
Keywords
Related Articles
- EP ID EP220709
- DOI -
- Views 169
- Downloads 0
Astrocytes in ischemic stroke – a potential target for neuroprotective strategies
Ischemic stroke is one of the leading causes of adult death and disability worldwide. Present applied therapeutic strategies do not give satisfactory results. It is often emphasized that pharmacological actions aimed at...
Właściwości biologiczne białek powierzchniowych bakterii z rodzaju Lactobacillus
Bakterie z rodzaju Lactobacillus to Gram-dodatnie bakterie, wśród których znajduje się wiele szczepów zakwalifikowanych do tzw. mikroflory probiotycznej. Probiotyki, jak wynika z definicji, to żywe mikroorganizmy, które...
Ferritin as a potential biomarker of efficacy of treatment of atrial fibrillation - preliminary report
<br/><b>Background</b>: Complex mechanisms of responsible for originating and maintaining of atrial fibrillation (AF) are involved in pathophysiology of this arrhythmia. Inflammation substantially contribute to arrhythmi...
Powiązania suchości skóry i świądu u chorych na przewlekłą chorobę nerek
Suchość skóry i świąd należą do najczęstszych zmian skórnych u chorych na przewlekłą chorobę nerek. Zmiany skórne są częstsze i o większym nasileniu w grupie dializowanych, niezależnie od rodzaju dializy, w porównaniu do...
Rola metaloproteinaz w modyfikacji macierzy zewnątrzkomórkowej w nowotworowym wzroście inwazyjnym, w przerzutowaniu i w angiogenezie
Metaloproteinazy macierzy zewnątrzkomórkowej (MMP) to rodzina endopeptydaz, które do aktywności proteolitycznej wymagają w centrum aktywnym obecności jonu cynku. Rodzinę MMP można podzielić na sześć grup: matrylizyny, ko...