Improved Differentiation of Mesenchymal Stem Cells into Hepatocyte-like Cells using FGF4 and IGF-1 in 3D Culture
Journal Title: International Journal of Stem Cell Research and Transplantation (IJST) - Year 2015, Vol 3, Issue 4
Abstract
Human Umbilical Cord Mesenchymal Stem Cells (UCMSCs) are considered as an excellent candidate for cell therapy to treat end-stage liver disease. Fibroblast Growth Factor-4 (FGF4), Hepatocyte Growth Factor, and Insulin-like Growth Factor-1 are some of the critical cytokines involved in liver development and regeneration. To evaluate the differentiation potency of cells into hepatocyte-like cells we used these cytokines. UCMSCs were isolated from Wharton's jelly of fullterm infants. The cells were characterized as MSCs by flow-cytometry and their multilineage differentiation capacity. Then, UCMSCs were cultured in 3D collagen scaffold and hepatogenic media with or without FGF4 for 21 days and the data were compared to control. The expression of liver specific genes was evaluated by real-time quantitative RT-PCR and immunocytochemistry. These cells expressed MSC markers and could differentiate into adipocytes and osteocytes. A non–significant higher level of liver specific genes, such as cytokeratin-18 and 19, alpha-fetoprotein and albumin, and also a significant higher level of CYP2B6 expressed by UCMSCs in hepatogenic medium containing FGF4 compared with control. In some specimens, cytokeratin-19-positive cells surrounded a luminal space within collagen scaffolds. Liver-specific marker expression was increased by pre-exposing the cells to FGF4 before treating with IGF-1 and HGF in 3D collagen scaffold.
Authors and Affiliations
Tahereh Talaei-Khozani
Keywords
Related Articles
- EP ID EP197084
- DOI 10.19070/2328-3548-1500018
- Views 110
- Downloads 0
Central Pontine Myelinolysis Associated with Cyclosporine in a Patient with NK Cell Leukaemia after Hematopoietic Stem Cell Transplantation
Central pontine myelinolysis (CPM) has been described as a rare complication of liver transplantation. Cases in allogeneic hematopoietic stem cell transplant are less reported. We present a patient with NK cell leukaemia...
Obtaining Mesenchymal Stem Cells From Adipose Tissue Of Murin Origin: Experimental Study
The aim of this study was to isolate and characterize rat Adipose Derived Mesenchymal Stem Cells (AD-MSCs) in order to evaluate their proliferative potential and their ability to differentiate in different cell types. AD...
From Precursor/Stem Cells To Cordocytic Phenotypes In The Skin
This cytohistopathological study was performed to have a better knowledge of the continuum of cellular events, from mesenchymal stem cells to mature cordocytic phenotypes with their morphological heterogeneity and multip...
Bioscaffold Augmentation of the Alveolar Bone: A Pilot Study in the Rabbit to Define the Protocol
Purpose: The current study aims to establish, for the first time, a model to determine the surgical and tissue processing protocols for testing the use of a synthetic bone graft material to augment the contour and volume...
Intravitreal Bevacizumab Followed by Ranibizumab for Neovascular Age Related Macular Degeneratıon
Purpose: To compare the outcomes after switching from intravitreal bevacizumab (IVB) to intravitreal ranibizumab (IVR) in patients with neovascular age related macular degeneration (ARMD). Material and Methods: A retrosp...