IN-SILICO DESIGN OF ANTIGENIC PEPTIDES AND MOLECULAR CLONING OF CYTOTOXIN ASSOCIATED GENE (cagA) OF H. PYLORI IN AN EXPRESSION VECTOR
Journal Title: International Journal of Pharmaceutical Sciences and Research (IJPSR) - Year 2019, Vol 10, Issue 6
Abstract
Helicobacter pylori (H. pylori) is a spiral, gram-negative pathogen which causes gastric cancer along with MALT lymphoma. It is widespread among the gut microflora and stands first among the pathogens. Cytotoxin-associated gene (cagA) is thought to be the vital protein in the pathogenesis of gastric cancer. Since there are no available vaccines at the moment, and owing to the increasing antibiotic resistance, studies towards developing a novel vaccine are very much essential. The present study was designed to clone and express the protein cagA within the bacterial systems. The protein fragment was screened for antigenic properties using in-silico studies. The protein of interest was confirmed using the western blots. This study further can be planned to produce a novel peptide vaccine.
Authors and Affiliations
Khamael Arif Mahdi Al-Challabi et al.
Keywords
Related Articles
- EP ID EP580654
- DOI 10.13040/IJPSR.0975-8232.10(6).2754-61
- Views 80
- Downloads 0
TOXICITY STUDIES OF A SAPONIN ISOLATED FROM THE FRUITS OF MOMORDICA DIOICA IN RATS
The objective of this study was to evaluate the toxicity of a saponin isolated from the fruits of the Momordica dioica as per OECD guidelines. Saponin was isolated from the methanolic extract of the fruits of M. dioica,...
PHARMACOGNOSTIC AND PHYTOCHEMICAL INVESTIGATION OF POTENTIALLY IMPORTANT PLANTS OF WESTERN GHATS, INDIA
Objective: The Indian traditional system of medicine, namely Ayurveda and Siddha, are primarily based on the use of the whole plant or different parts of plant singly or in combination to make multifactorial formulations...
MOLECULAR DOCKING STUDIES OF A FEW NOVEL PYRIMIDINE DERIVATIVES AS REVERSE TRANSCRIPTASE HIV-1 INHIBITORS
Anti-HIV drug discovery has been increasingly focusing on HIV-1- reverse transcriptase as a potential therapeutic target. Pyrimidine-based derivatives are studied for their HIV-1 reverse transcriptase inhibition activiti...
HYDROXYAPATITE AND Zn-HYDROXYAPATITE SYNTHESIS USING CALCIUM FROM LAKE MANINJAU PENSI SHELLS AND RESISTANCE TEST ON BACTERIA
HAp and Zn-HAp Synthesis with several concentrations of Zn (5%, 10%, 15%, and 20%) w/w using Ca precursor from Maninjau Lake pensi shells was done with the sol-gel method. XRF for three hours obtained 96.525% Ca content...
IMPACT OF HYDROTHERMAL ISOLATION METHOD ON THE RECOVERY OF FENUGREEK SEED HEMI-CELLULOSE ANALYZED THROUGH FTIR-DSC-SEM INTERPRETATIONS
Natural Herbal components like seeds mucilages, exudates, gums, polysaccharides, celluloses, hemicelluloses, starch, etc. are now a day’s exploited as excipients. Literature reveals medicinal, nutraceutical and pharmaceu...