In Silico MicroRNA Identification from Stevia rebaudiana Transcriptome Assembly
Journal Title: European Journal of Medicinal Plants - Year 2016, Vol 15, Issue 2
Abstract
MicroRNAs are a class of endogenous, approximately 22 nucleotides in length noncoding RNA, which is evolutionary conserved and mediates post-transcriptional gene regulation. MicroRNA play a crucial role in development of plant, cellular processes, biological processes, cell proliferation and stress response. Stevia rebaudiana is an economically important and medicinal plant of the Asteraceae family. A total of 1,418,58 unigenes from Stevia transcriptome data were used for homology search against known plant miRNA database miRBase version 21. The functionally annotated unigenes were excluded from the studies. Total 381 non-protein coding unigenes were considered for candidates of miRNA precursor in Stevia. One potential miRNA from miR168 family with secondary structure was identified through the sequel of stringent filtering criteria. The target prediction of novel miRNA was carried out for using psRNATarget program based on their sequence complementarities. A total of 31 potential gene targets were predicted for identified novel miRNA, which playing crucial role in various biological processes like development of plant, DNA repair, splicing, post-translational gene silencing, plant defense response, cell growth and proliferation. The phylogenetic analysis was also carried out to study the conserved nature of miRNA. These findings provide significant insights of miRNA and their potential role in Stevia as well as their regulatory mechanisms.
Authors and Affiliations
Aditya Mehta, Hemant Gupta, Rakesh Rawal, Archana Mankad, Tanushree Tiwari, Maulik Patel, Arpita Ghosh
Keywords
Related Articles
- EP ID EP349640
- DOI 10.9734/EJMP/2016/25221
- Views 92
- Downloads 0
Aqueous Extract of the Fruits of Xylopia aethiopica (Dunal) A. Rich. Protects against Carbon Tetrachloride - Induced Hepatotoxicity in Rats
Aim: This study was designed to examine the hepatoprotective effect of aqueous extract of the fruits of Xylopia aethiopica using the carbon tetrachloride (CCl4) model. Place and Duration of Study: Department of Chemical...
In vitro Antimycobacterial Activity of Sterculia quinqueloba (Garcke) K. Schumand Canthium crassum Hiern
Aim: To screen for the anti-mycobacterial activity of Canthium crassum and Sterculia quinqueloba using two mycobacteria species the Mycobacteria madagascariense and Mycobacteria indicuspranii. Study Design: In vitro assa...
In vitro Shoot Multiplication of Medicinally Important Plant Andrographis lineata Nees via Nodal Explants
Andrographis lineata Nees (Acanthaceae) is an endemic herb rich in flavonoids and diterpenoids which have pharmaceutical importance. Seedling explants source has not been studied for micropropagation and hence the presen...
Antioxidant and in vivo Wound Healing Activities of Clausena anisata
Leaves of Clausena anisata are used in many parts of West Africa including Ghana for management of wounds and other skin infections. The study was to evaluate the in vivo wound healing and antioxidant properties of ethan...
In vitro Antioxidant, Antimicrobial, Insecticidal and Cytotoxic Activities of the Medicinal Plants: Allamanda cathartica and Mimusops elengi
A comparison of in vitro antioxidant, antimicrobial, insecticidal and cytotoxic activities of two medicinal plants, Allamanda cathartica and Mimusops elengi have been studied by spectrophotometric and disk diffusion meth...