In Vitro and In Vivo Effectiveness of GPK/B2O, A Phytocomplex in Two Experimental Flu Virus Models
Journal Title: Open Access Journal of Pharmaceutical Research - Year 2021, Vol 5, Issue 1
Abstract
There is constant quest to prevent and counterfight annual influenza viral epidemics, given the limitations and drawbacks of antivirals chemicals so far. The aim of the present study was to test on an experimental viral model a phytocompound containing herbal ingredients endowed by, albeit scattered, reports of antiviral properties (GPK/B20, Viraxil, Named, Italy). Two influenza models were employed, i.e. H1N1 and H3N2. In vitro test showed that GSK/B20, especially at medium and high dose (50µl/mL to 100µl/mL) significantly reduced the infection of Madin-Darby-Canine-Kidney monolayers cells and progression of cytopathogenic effect over the observation period. More interestingly, when this compound was test 1h after infection on mice, especially at high dose (60g/kg/day), it appeared to significantly blunt the immune/inflammatory cells recruitment and the inflammatory cytokine cascade (at lung tissue level and bronco-alveolar lavage level), curbing also the related gene expression. This was associated to a reduced lung oedema, which is the correlate of respiratory distress in humans, viral load and scanning electron microscopy findings. In the meantime, more detailed experimental studies are warranted to unveil the range of mechanisms of actions that these natural ingredients are likely to be endowed with. Although these data have to be taken with caution having being obtained in an experimental-controlled setting, they suggest a potential safe application in the clinical practice, as recently corroborated for one of the herbal used.
Authors and Affiliations
Rastmanesh R, Kushugulova A, Kumar N, Nagyzbekkyzy E, Rasulova S, Lorenzetti A and He F*
Keywords
Related Articles
- EP ID EP748529
- DOI 10.23880/oajpr-16000228
- Views 31
- Downloads 0
The Utilization of Residue Come Out as a By-Product by the Cold Press
In recent years, with the increase of the world population, the studies on the utilization of the herbal wastes resulting from increased industrialization and production have gained speed and importance. Vegetable oils t...
Validating the Selection Process of Healthy Control Group Based on Apparently Healthy Volunteers
Objectives: The objective of this cross sectional study is to evaluate the probability of finding healthy control subjects according to the results of multiple lab tests in multiple domains including biochemical hematolo...
Marine Algae as a Precursor for Eco-Friendly Synthesis of Nanoparticles
In the last decade, researchers were developing different types of nanoparticles and exploring more and more applications that made this area of research so promising and very active. Actually, many techniques can be fo...
Tamoxifen: Pharmacokinetics and Pharmacodynamics
Tamoxifen is the hormone therapy of choice in pre-menopausal estrogen receptor positive breast cancer in women and sometimes in post-menopausal women. The environmental and genetic factors widely affect the pharmacokinet...
Drug-Related Problems in Hypertensive Patients with Multiple Comorbidities
Hypertensive patients with multiple comorbidities often received multiple medications to achieve target blood pressure and to treat other comorbidities which can lead to the occurrence of drug-related problems (DRP). The...