IN VITRO ANTI-LEISHMANIAL ACTIVITY AGAINST CUTANEOUS LEISHMANIA PARASITES AND PRELIMINARY PHYTOCHEMICAL ANALYSIS OF FOUR YEMENI MEDICINAL PLANTS
Journal Title: Universal Journal of Pharmaceutical Research - Year 2018, Vol 3, Issue 4
Abstract
Cutaneous leishmaniasis is one form of leishmaniasis that chiefly infected the poor sections of the society. The prototypical therapeutic interventions in vogue are handicapped due to toxicity and an alarming increase in drug resistance. Furthermore, the absence of vaccines has raised the quest for alternative therapies. So, the aim of our study was to assess the anti-leishmanial activity of Euphorbia cactus Ehrenb, Euphorbia ammak Forssk, Euphorbia inarticulate Schweinf, and Pergularia tomentosa L. The extracts of plants were prepared by maceration method and by Soxhlet extractor. The extracts were dried and re-dissolved in 2% dimethyl sulfoxide (DMSO) 1% solvent. Leishmania spp. cells were then tested with serial concentrations (15.6 μgml-1 to 500 μg ml-1 ) of the extracts, using the 3-(4,5-dimethylthazolk-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. All experiments were performed in triplicate and analyzed by ANOVA test. The optical density values as measured by Enzyme-Linked Immunosorbent Assay (ELISA) were used to calculate the IC50 values. The results indicated that the methanolic latex extract of Euphorbia cactus Ehrenb, Euphorbia ammak Forssk had potent anti-leishmanial activity against the promastigotes of Leishmania spp. based on a dose-dependent response analysis. The IC50 values for Euphorbia cactus Ehrenb and Euphorbia ammak Forssk after 24h incubation against Leishmania spp. promastigotes were less than <15.6 μgml-1. Furthermore, the phytochemical analysis of methanolic extracts showed the presence of alkaloids, phytosterols, phenols, saponins, and flavonoids in which these components have been proven previously to be the active compounds against Leishmania parasite. In conclusion, the present study reveals that latex extract of Euphorbia cactus Ehrenb and Euphorbia ammak Forssk contain active compounds that have anti-leishmanial activity, which could serve as an alternative agent in the treatment of Cutaneous leishmaniasis, but further studies would, therefore, be needed to assess the activity of these materials of this plants in vivo clinical response and study their toxicity on cell lines.
Authors and Affiliations
Manal Mutahar Ali Al- Hajj, Hassan A Al-Shamahy, Bushra Y Alkhatib, Bushra A Moharram
Keywords
Related Articles
- EP ID EP36481
- DOI https://doi.org/10.22270/ujpr.v3i4.183
- Views 340
- Downloads 0
A REVIEW OF BREAST CANCER RISK FACTORS
Breast cancer is a commonly diagnosed neoplastic ailment in females particularly near menopause. This ailment signifies a substantial health problem as it has influenced a large number of women. Several risk factors are...
PATTERN OF ANTIMICROBIAL PRESCRIBING AMONG IN-PATIENTS OF A TEACHING HOSPITAL IN YEMEN: A PROSPECTIVE STUDY
The irrational use of antimicrobials leads to a number of consequences in term of cost, drug interactions, hospital stay and bacterial resistance, and a substantial economic burden on health care systems. This study aime...
IN VITRO ANTI-LEISHMANIAL ACTIVITY AGAINST CUTANEOUS LEISHMANIA PARASITES AND PRELIMINARY PHYTOCHEMICAL ANALYSIS OF FOUR YEMENI MEDICINAL PLANTS
Cutaneous leishmaniasis is one form of leishmaniasis that chiefly infected the poor sections of the society. The prototypical therapeutic interventions in vogue are handicapped due to toxicity and an alarming increase in...
TABLET GRANULATION: CURRENT SCENARIO AND RECENT ADVANCES
Granulation is a size enlargement process, in fine or coarse particles converted into physically stronger and larger agglomerates having good flow property, better compression characteristics and uniformity, prevent segr...
MURRAYA KOENIGI-A BOON IN DIFFERENT PATHOLOGICAL CONDITIONS
Since very long period of time medicinal plants or their bioactive compounds have been utilized by majority of world population particularly in developing countries for primary and traditional healthcare system. At prese...