IN VITRO ANTIOXIDANT ACTIVITY AND TOTAL PHENOLIC CONTENT OF LEAF EXTRACTS OF DALBERGIA SISSOO (ROXB.)
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2016, Vol 7, Issue 8
Abstract
Oxidative stress is the state of imbalance between the level of antioxidant defense system and production of reactive oxygen species. Antioxidants are an ancillary defense against oxidative stress. Natural antioxidants such as polyphenols and flavonoids present in the plants scavenge harmful free radicals from our body. The aim of the present study was to determine total phenolic content and in-vitro antioxidant activity in methanolic and ethanolic leaf extract of Dalbergia sissoo known for its medicinal properties in folk medicine. The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. The Antioxidant activity was compared with ascorbic acid as standard antioxidant. Quantitative analysis of antioxidative components like total phenolic content was determined by spectrophotometric method and expressed in gallic acid equivalent. The ethanol extract had the highest total phenolic content followed by methanol. The ethanolic crude extracts showed very promising antioxidant activity compared with methanolic extract. The EC50 value for ethanolic extract was found to be 106.3μg/ml. The total phenolic content was found out to be 0.185±1.87mg/gm GAE. The findings of the present study suggested that polyphenols present in Dalbergia sissoo extract may be responsible for the antioxidant activity. Dalbergia sissoo (Roxb.) could be a potential natural source of antioxidants and could have greater importance as therapeutic agent in preventing or slowing oxidative stress related degenerative diseases. Further investigations need to be carried out to isolate antioxidant compounds present in the plant extract and evaluate in vivo antioxidant activity.
Authors and Affiliations
Nikita Rijhwani, Rajkumar Bharty
Keywords
Related Articles
- EP ID EP144144
- DOI 10.7897/2230-8407.07891
- Views 61
- Downloads 0
PROTECTIVE EFFECT OF SPIRULINA ON ETHYLENE GLYCOL INDUCED UROLITHIASIS IN RATS
The aim of our present study is to assess the effects of spirulina as a preventive agent in experimentally induced urolithiasis model in rats. Rats were induced to produce kidney stone by providing drinking water contain...
PRELIMINARY PHYTOCHEMICAL EVALUATION OF WHOLE PLANT EXTRACT OF DIPTERACANTHUS PROSTRATUS NEES
Dipteracanthus prostratus is an prostrate perennial herb. It is a small straggling, much branched herb it is purple at the nodes, internodes are long and hairy. It used as anti-cancer, hypoglycemic, anti-inflammatory and...
SPHERICAL AGGLOMERATION – DIRECT TABLETTING TECHNIQUE
Direct tabletting technique is the modern and the most efficient process used in tablet manufacturing and has been successfully employed for various poorly soluble and poorly compressible drugs. Spherical agglomeration...
ANTITUBERCULOSIS AND PHYTOCHEMICAL INVESTIGATION OF THE DICHLOROMETHANE EXTRACT PLEUROTUS TUBER-REGIUM (FRIES) SINGER SCLEROTIUM
Incidence of tuberculosis infections is on the rise. The cost and length of orthodox treatment regimen coupled with the rise in multidrug resistance cases make the development of more effective and relatively cheaper alt...
LIFE STYLE RELATED HEALTH HAZARDS
Our lifestyle is determined by our normal patterns of living. Life has become fast, ambitions and materialistic now-a-days. Routine day to day activities such as continuous use of Mobiles, long hours at computers/televis...