IN VITRO ANTIOXIDANT AND ANTICANCER ACTIVITY OF MIMOSA PUDICA LINN EXTRACT AND L-MIMOSINE ON LYMPHOMA DAUDI CELLS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 12
Abstract
Objective: The present study is an attempt to investigate the antioxidant and anticancer potential of hydroalcoholic extract of Mimosa pudica Linn (Mimosaceae) and L-Mimosine on Daudi cell line.Methods: The analysis of the standard compound L-Mimosine was ascertained by High Performance Thin Layer Chromatography (HPTLC). Free radical scavenging activity of M. pudica extract and L-Mimosine was also compared using 2, 2-diphenyl–1-picrylhydrazylradical scavenging assay (DPPH). Cell viability and cytotoxicity on Daudi cells were evaluated by trypan blue and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays in a dose and time-dependent manner respectively.Results: HPTLC analysis showed the presence of amino acids, amines, lipids in the hydroalcoholic extract of M. pudica. Crude hydroalcoholic extract of M. pudica showed antioxidant activity (IC50=103.88 µg/ml) whereas L-Mimosine showed antioxidant activity (IC50=233.06 µM). The crude extract and compound inhibited the proliferation and growth of the Daudi cells through induced cell death. The IC50 value for anticancer activity was found to be 201.65 µg/ml and 86.61 µM at 72 h for M. pudica extract and L-Mimosine respectively.Conclusion: The results indicated the presence of L-Mimosine in the hydroalcoholic extract of M. pudica. The hydroalcoholic extract and pure compound proved potent inhibitors of cell proliferation, thus manifesting significant antioxidant and anticancer activity.Â
Authors and Affiliations
Felisa Parmar, Nisha Kushawaha, Hyacinth Highland, Linz-buoy George
Keywords
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