IN VITRO PHOTO-TOXICITY SCREENING OF HYDROALCOHOLIC EXTRACT OBTAINED FROM MANILKARA ZAPOTA (L.) ROYEN LEAVES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 11
Abstract
Objective: Cosmetic products like sunscreens are being used widely nowadays due to increasing consumer awareness about the harmful effects of ultraviolet light exposure. Conventionally used chemical filters lack photostability hence produce skin reactions. The present research thus aims to evaluate the photostability of the hydro-alcoholic extract obtained from the leaves of Manilkara zapota (L.) Royen, Sapotaceae, a potential sunscreen using in vitro phototoxicity assays.Methods: Leaves of the plant was extracted using hydroalcoholic solvent (water: ethanol, 3:7) by maceration technique. Candida albicans lawn seeded Sabouraud dextrose agar plate, impregnated with test extract soaked filter paper disc was irradiated at a dose 0.2 mW/cm2 for 4 hours. Absorption spectra of known concentration of the test extract at initial as well on irradiation was measured and compared with standards. Linoleic acid peroxidation test was used to determine the extent of peroxides formed using TBARS assay.Results: Candida yeast test revealed that there was no photodegradation metabolites of the extract formed on irradiation thus growth of the Candida albicans remained unaffected. Spectra measurement studies revealed no significant change in the spectrum in the wavelength 250-320 nm of M. zapota extract however the standard phototoxic compound showed a significant change. Linoleic acid peroxidation test revealed a very limited peroxide formation 6.47 μmoles/ ml comparable with the standard Quercetin, Gallic acid and marketed sunscreen formulation.Conclusion: All the in vitro phototoxicity assays concluded that the hydroalcoholic extract obtained from Manilkara zapota (L.) Royen leaves exhibited photostability to the Ultraviolet irradiation.Â
Authors and Affiliations
Norma Leonard Rebello, Suhasini Shyamkant Bhalerao
Keywords
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