In-vitro callus regeneration and rhizogenesis in Diploknema butyracea: a valuable tree borne oilseed
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2017, Vol 5, Issue 3
Abstract
In-vitro callus regeneration and rhizogenesis is achieved from leaf segments of mature tree of Diploknema butyracea (Roxb.) Lam. Explants sterilization was standardized using 0.1% HgCl2 for 3 minutes and NaOCl (40%) for 5 minutes. High frequency callus was regenerated in MS medium with 4.44 µM BAP + 2.26 µM 2, 4-D. Maximum rhizogenesis was recorded in MS medium with 4.44 µM BAP + 1.14 µM IAA + 0.58 µM GA3. Callus culture failed to produce shoot in all tried combination of PGRs in MS medium.
Authors and Affiliations
Arti Kunwar and Ajay Thakur
Keywords
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