In Vitro Chemosensitivity Assessed with MTT Assay in 100 Leukemic Patients
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 15, Issue 2
Abstract
Chemotherapy possesses an important position in the treatment course of cancer. Cel-lular drug resistance is supposed to play a major role in chemotherapy failure. The purpose of this study is to investigate the feasibility of the application of MTT assay in the in vitrochemosensitivity test, in attempt to supply theory reference for clinical therapy of leukemia. We obtained peripheral blood from 100 patients with leukemia at the time of initial diag-nosis or relapse. Leukemic cells were separated and the chemosensitivity testing was per-formed by MTT colorimetric assay. The following drugs were tested: cytarabine, vincristine, methotrexate, daunorubicin, etoposide, teniposide, homoharringtonine, doxorubicin, and mitoxantrone. The Inhibition rates of each drug for four kinds of leukemia were analyzed. Abbreviations: DNR: Daunorubicin; VP-16: Etoposide; Ara-C: Cytosine Arabinoside; VCR: Vincristine; HHT: Homoharringtonine; MIT: Mitoxantrone; DOX: Daunomycin; VM-26: Ten-iposide; MTX: Methotrexate. ALL: Acute Lymphocytic Leukemia; ANLL: Acute Non Lympho-cytic Leukemia; CLL: Chronic Lymphocytic Leukemia; CML: Chronic Myelogenous Leukemia
Authors and Affiliations
Zhi guo Zhang, Jie Song, Shuai Bai, Hua Yang
Keywords
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- EP ID EP591728
- DOI 10.26717/BJSTR.2019.15.002665
- Views 163
- Downloads 0
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