In vitro cytotoxic studies of various formulations of Indian medicinal plants
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2015, Vol 3, Issue 3
Abstract
The present study explores the cytotoxic activity of various polyherbal formulations prepared from the combination of chloroform extracts of leaves of Tevetia peruviana, Waltheria indica and Toddalia asiatica. Polyherbal formulations were formulated and assessed for their cytotoxic activity by using MTT assay by comparing with Curcumin as standard. The highly toxic formulation (F9) were further assayed for Cell viability by using Trypan blue dye exclusion assay by comparing with Doxorubin as standard, DNA damage protection by DNA fragmentation assay. The morphology of apoptotic cell and nucleus was identified by employing Acridine orange and Ethidiun bromide staining and Hoechst staining.MTT assay showed the dose dependent response with concentrations ranging from 62.5 to 1000 µg/ml with overall CTC50 values ranging from 20.00 ±3.0 to 1000.Among the different formulations F3,F4,F5,F9 showed high toxicity and F1,F2,F6,F10 showed low toxicity.Cell viability by Trypan blue dye exclusion showed a remarkable growth rate decrease to 50% in the number of treated cells when compared to control cells after 24 hrs while significant decrease was observed at 500 μg/ml concentration. DNA of the cells treated with 100-1000 μg/ml concentrations induced the internucleosomal breakdown of chromatin DNA, resulting in ladder-like agarose electrophoretic patterns of degraded DNA products, typical of classical apoptosis. From these results it may be concluded that the F9 formulation demonstrated significant protective activity that might be due to combined effect of all these extracts.
Authors and Affiliations
Sirimal Gopi Krishna
Keywords
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