In vitro establishment of Heliconia rostrata Ruiz & Pavón and Heliconia wagnerina Petersen
Journal Title: Biotecnología Vegetal - Year 2013, Vol 13, Issue 4
Abstract
The heliconia have wide appeal as ornamental plants and their environmental significance. The species Heliconia rostrata and H. wagneriana are planted in the Botanical Garden of Cienfuegos, Cuba. The aim of this study was to in vitro establish explants of these species to initiate propagation through biotechnological methods. Rhizomes were used as initial explants and were disinfected with sodium hypochlorite. In H. rostrata two concentrations of sodium hypochlorite (v/v) (2% and 5%) were tested for 5 and 10 min. In H. wagneriana two concentrations were tested (3% and 5%) for 15 and 25 min. The numbers of explants contaminated with microorganisms, the number of live explants and sprouted were quantified. Microbial contamination losses affected the explants survival. With the use of 5% sodium hypochlorite better results was obtained. Through the use of these procedures it was possible the in vitro establishment of both species of heliconia.
Authors and Affiliations
Flora Margarita Sosa Rodríguez , Alexander Díaz López , Yanelis Puerta González , Enrique Casanovas Cosío , Roberto Suarez Surí , Odalmys Pérez Espinosa , Marilín Fontes Leandro
Keywords
Related Articles
- EP ID EP683674
- DOI -
- Views 138
- Downloads 1
In vitro establishment of apex and nodule segments of Swietenia macrophylla King
The application of tissue culture techniques in woody plants offers a valuable alternative for the propagation of elite trees. With the objective of obtaining the in vitro establishment of mahogany (Swietenia macrophyll...
Effect of carbendazim for the control of Colletotrichum sp., Contaminant of the in vitro establishment of coffee callus
Collectotrichum genus was identified as frequent contaminant in coffee callus formation from foliar explants fragments of plants cultivated in field. The presence of this genus as contaminant in fragments of coffee leave...
Automated system for counting and sorting cells in sugarcane suspensions
The quantity and types (embryogenic and non-embryogenic) of cells are very important parameters to evaluate plant cell suspension growth for determine the best suspension to obtaining plant regeneration by somatic embryo...
Effect of the planting material on the incidence of dry rot in Colocasia esculenta (L.) Schott and Xanthosoma spp.
The cocoyam (Xanthosoma spp. and Colocasia esculenta) is a monocot plant with vegetative propagation. This work was developed with the aim of determining the effect of the planting material type on the incidence of dry...
Management of suckers and apices of cultivars of Musa spp. to initiate micropropagation and behavior during six in vitro subcultures
Two forms of handling of the tillers were evaluated to extract the apexes that will be established in vitro. One was the method of the planting the rhizomes which below to the small tiller in the concrete beds, to cause...