In vitro establishment of Jatropha curcas L. from different types of explants
Journal Title: Biotecnología Vegetal - Year 2012, Vol 12, Issue 4
Abstract
The use of Jatropha curcas L. for biofuels production is growing rapidly worldwide. Traditional methods of propagation have failed to respond to that demand. In vitro culture could become an alternative to propagate this species on a commercial scale. This study aimed to achieve the in vitro establishment of different progenies of J. curcas from various explant types. For this study, apical bud, axillaries buds and leaf discs were used and treated with different concentrations of sodium hypochlorite for 15 minutes. In the case of the apical and axillaries buds not observed fungal contamination but bacterial contamination ranged between 16 and 33%. A concentration of 3.0% NaOCl increased tissue necrosis and caused mortality to the 21% of the explants, while a 2.0% increased sprouting of the apical (83.3%) and axillaries (54%) buds. For leaf discs the best results in terms of microbial contamination rate (30%) and callus formation (65%) were obtained with 1.5% NaOCl. The results give a procedure that guarantees the in vitro establishment of different types of explants, which is a starting point in developing a protocol for the in vitro propagation of this species.
Authors and Affiliations
Maité Chávez , Manuel de Feria, Mariana La O, Leonardo Rivero
Keywords
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