In vitro propagation of Hedeoma multiflora Benth. native germplasm via organogenesis
Journal Title: Biotecnología Vegetal - Year 2018, Vol 18, Issue 2
Abstract
The natural populations of Hedeoma multiflora Benth. (Lamiaceae) are overexploited and endangered due to their multiple uses. It is necessary to implement propagation methods of native germplasm to achieve the sustainability of the plant resource. The objective of the present work was to in vitro propagate H. multiflora native germplasm via organogenesis. Binodal segments of plants grown under natural conditions were in vitro established in 50% Murashige and Skoog medium with 2.69 μM naphthaleneacetic acid (NAA) and 2.22 μM benzylaminopurine (BA). The shoots obtained were individualized and subcultured to 50% MS with different concentrations of NAA and BA. The in vitro plants were acclimatized in four substrates: perlite, perlite: soil (1:1), perlite: peat (1:1) and soil. With the experimental conditions tested, the in vitro establishment of H. multiflora was possible. No significant differences were observed for the number of shoots per explant with the combinations of growth regulators used. In the ex vitro acclimatization stage, a survival between 40 and 80% was obtained, and then decreased below 20%. The in vitro propagation of H. multiflora native germplasm via organogenesis generates a high number of in vitro plants. These could be used to restore damaged ecosystems, the germplasm conservation and the production of secondary metabolites.
Authors and Affiliations
María Soledad Díaz Gabutti, Jorge Leporati, Patricia Verdes
Keywords
Related Articles
- EP ID EP682961
- DOI -
- Views 141
- Downloads 1
Effect of light and gibberellic acid on in vitro germination of Capsicum annuum L. cv. ‘Papri King’
In the coastal zone of Peru, paprika (Capsicum annuum L.) is a crop of great importance, but it has a high production cost because the seeds are germinated in seedlings and this stage is considered crucial. The objecti...
Effect of carborundum damage on regeneration and expression a-glucuronidase transient in different explants of Phaseolus vulgaris var. CIAP 7247
Agrobacterium tumefaciens-mediated genetic transformation is difficult in beans. Efficiency can be improved preparing explants for the infection. The aim of this research was to determine the effect of treatment with c...
Protocol for isolation of Moniliophthora roreri (Cif and Par) Evans et al. from cacao fruits cv. ‘National’ in the Ecuadorian Amazonia
Moniliophthora roreri is a pathogen of cocoa (Theobroma cacao L.) that causes high economic losses in Ecuador. This paper is intended to present a protocol for the isolation of the pathogen from cocoa fruits cv. ‘Natio...
In vitro multiplication of ‘FHIA-25’ (Musa spp., AAB) in Systems Temporary Immersion
Necessity to produce high quality planting material has required searching new alternatives to increase the efficiency of in vitro propagation methods and their automation, as in Temporary Immersion Systems (TIS). This...
Novel explant for somatic embryogenesis in Sorghum bicolor (L.) Mohen
This work was carried out with the objective to form somatic embryos of sorghum, cv. ‘CIAP 132R-05’ starting from callus obtained from sections of in vitro shoots. For the formation of callus, different concentrations o...