Somatic embryogenesis in bananas and plantains from immature male flowers
Journal Title: Biotecnología Vegetal - Year 2001, Vol 1, Issue 1
Abstract
In the development of this work, aspects related to the achievement of somatic embryos and regeneration of plants in some cultivars of Musa sp. group AAA, ABB and AABB were studied. Different experiments were carried out to determine the optimum culture conditions; the solidifying agent resulted to be phytagel (SIGMA Co.), at a concentration of 2.0 g.l -1 , were 68.6% of the explants formed yellow nodular calli. With, respect to the incubation conditions, darkness had a better influence achieving 73.8% of the explants forming yellow nodular calli. The best dosage of 2,4-D could have been determined for the induction of the yellow nodular calli in each one of the cultivars studied: Grande Naine (AAA), Parecido al Rey (AAA) and FHIA-03 (AABB) 4.0 mg.l -1 and for Bluggoe (ABB) 2.0 mg.l -1 . Studying the ranges of the hands, it was found that for all the cultivars, the ranges from 5-9 formed more callus with somatic embryogenesis of high frequency than the ranges from 10-14. Once the somatic embryos were transferred to the germination culture medium, a rate of 48.0% was obtained for Grande Naine and 39.0% for Parecido al Rey.
Authors and Affiliations
Rafael Gómez-Kosky, Luis del Sol, Maritza Reyes, Marisol Freire-Seijó, Laisyn Posada-Pérez, Idalia Herrera, Jean Vicent Escalant
Keywords
Related Articles
- EP ID EP697705
- DOI -
- Views 47
- Downloads 0
Response of Phaseolus vulgaris L. cultivars to drought stress
Tolerance to drought is a desired cultivars of Phaseolus vulgaris L. The present study aimed to determine the response of 22 cultivars of common bean during the early stages of vegetative development character. The pla...
Use of Temporary Immersion Systems in the multiplication and germination of somatic embryos of banana (Musa sp.) Cultivar Gran Enano (AAA) and papaya (Carica papaya L. var. Maradol Rojo)
The method consisted of sowing embryogenic culture in Filtration Systems Sartorius autoclaveable of 250 ml of capacity. Different densities inoculum that varied in dependency of the culture were used(250 mg for banana...
Multiplication and germination of somatic embryos in Cuban varieties of soy Incasoy-1 and Incasoy-27
Soybean is genotype-dependent as it has been shown. This has lead to work on new protocols for plant regeneration. This research aimed to achieve growth and germination of somatic embryos in the Cuban varieties Incasoy...
Risk assessment of Horizontal Gene Transfer in bacterial contaminants of sugarcane in vitro cultures
The horizontal gene transfer is a phenomenon that must be taken into account when performing risk evaluation of genetically modified plants, because these ones could transmit by this method the artificially incorporated...
Isolation of a fragment of the ACC Oxidase cDNA expressed during the ripening of the Guava Enana fruit
Isolation and cloning of a cDNA fragment encoding ACC oxidase from ripe guava fruits by RT-PCR is shown in this report. It is the early step to apply RNA antisense technology for gene silence in order to control guava fr...