Somatic embryogenesis in bananas and plantains from immature male flowers
Journal Title: Biotecnología Vegetal - Year 2001, Vol 1, Issue 1
Abstract
In the development of this work, aspects related to the achievement of somatic embryos and regeneration of plants in some cultivars of Musa sp. group AAA, ABB and AABB were studied. Different experiments were carried out to determine the optimum culture conditions; the solidifying agent resulted to be phytagel (SIGMA Co.), at a concentration of 2.0 g.l -1 , were 68.6% of the explants formed yellow nodular calli. With, respect to the incubation conditions, darkness had a better influence achieving 73.8% of the explants forming yellow nodular calli. The best dosage of 2,4-D could have been determined for the induction of the yellow nodular calli in each one of the cultivars studied: Grande Naine (AAA), Parecido al Rey (AAA) and FHIA-03 (AABB) 4.0 mg.l -1 and for Bluggoe (ABB) 2.0 mg.l -1 . Studying the ranges of the hands, it was found that for all the cultivars, the ranges from 5-9 formed more callus with somatic embryogenesis of high frequency than the ranges from 10-14. Once the somatic embryos were transferred to the germination culture medium, a rate of 48.0% was obtained for Grande Naine and 39.0% for Parecido al Rey.
Authors and Affiliations
Rafael Gómez-Kosky, Luis del Sol, Maritza Reyes, Marisol Freire-Seijó, Laisyn Posada-Pérez, Idalia Herrera, Jean Vicent Escalant
Keywords
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