Germination of somatic embryos of Swietenia macrophylla in semi-solid culture media
Journal Title: Biotecnología Vegetal - Year 2005, Vol 5, Issue 1
Abstract
With the objective to establish a suitable culture medium and to determine the optimum culture time for the germination of somatic embryos of Swietenia macrophylla King, it was carried out these work. Somatic embryos at the cotyledonal stage were placed in a culture medium formed by the half of the MS salts for their germination . three concentrations of 6-BAP (0.2, 0.4 and 0.6 mg.l -1 ), two concentrations of AG (1.0 and 2.0 mg.l -1 ) and a culture medium control without 3 growth regulators were studied. In the culture medium where the best results were obtained, the effect of the culture time on the germination of somatic embryos (30, 60 and 90 days of culture) was studied. The major number of somatic embryos with total germination was obtained in the culture medium without growth regulators, with significant differences to the rest of the treatments. The highest value of somatic embryos with secondary embryogenesis was presented when 0.6 mg.l -1 of 6-BAP were added to the culture medium. The increase of the culture time to 60 and 90 days increased significantly the total germination of the somatic embryos. These results allowed to define a culture medium for the germination of somatic embryos of Swietenia macrophylla King composed by the half of the MS salts without growth regulators with a culture time of 60 days.
Authors and Affiliations
Raúl Collad*, Raúl Barbón, Daniel Agramonte, Felipe Jiménez, Martha Pérez, Odalys Gutiérrez
Keywords
Related Articles
- EP ID EP684213
- DOI -
- Views 127
- Downloads 0
Optimization of the culture medium and fermentation conditions for Azotobacter chroococcum based biofertilizers production
In Cuba, a biofertilizer has been developed based on a nitrogen fixer Azotobacter chroococcum strain. This work aimed to optimize the culture medium and the fermentation conditions for industrial scale. First, tests in t...
Evaluation in two agroecosystems of the behavior of papaya vitroplants cv Maradol roja
Papaya plants (Carica papaya L.) cv Maradol Roja, obtained by in vitro culture (3rd and 12th subculture), and control plants spread by the traditional way, were planted in two agroecosystems. When evaluating the obtained...
Histological characterization of the sugarcane response to Puccinia kuehnii infection
Sugarcane orange rust, caused by Puccinia kuehnii (W. Krüger) EJ Butler, is one of the major diseases impacting sugarcane Saccharum spp. production worldwide. The objective of this work was to characterize by histology...
Effects of different types of seed in Agave fourcroydes Lem. variety ‘Sac Ki’ propagation over indicators related to the growth in nursery
A study was conducted during 15 months under nursery conditions, with the objective to evaluate the effect of different types of seed (in vitro grown plants (V), bulbils of in vitro plants (BV), rhizomes of in vitro pla...
Use of PECTIMORF and BIOBRAS-16 in somatic embryogenesis of potatoes
With the application of PECTIMORF and BIOBRAS-16, somatic embryos were obtained in potato (Solanum tuberosum, L) c.v. Desirèe, of 40 days old callus obtained from stem micropropagated plants. These were used as possible...