In vitro Growth Inhibition Study of Urinary Type Brushite Crystals in the Presence of Healthy Adult Urine and Tartaric acid
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 4
Abstract
: Brushite or calcium hydrogen phosphate dehydrate is commonly found in urinary stones. In the present study the single diffusion gel growth technique was used to grow brushite crystals in silica hydro gel medium. It provides controlled environment to simulate the growth of brushite containing urolithiasis under static conditions in the presence of healthy human urine. In this in vitro study, the supernatant solution containing calcium chloride was used to grow brushite crystals and considered as control. To study the effect of urine on the growth of brushite crystals, different volume of healthy human urine was added in the supernatant solution. In this in vitro study, the role of healthy human urine on the growth of brushite crystals in terms of effect of the pH of supernatant solutions and volume of urine on the morphology, size and number of grown crystals were studied. The inhibition effect of tartaric acid was verified by adding tartaric acid to urine and calcium chloride containing supernatant solution. Tartaric acid was selected because tamarind contains tartaric acid and quite frequently it is used in food preparations. Tartaric acid as well as natural urine both was found to be good inhibitors of brushite crystal growth. The addition of tartaric acid along with urine in the supernatant solution stopped the nucleation of brushite crystals. Results are discussed.
Authors and Affiliations
*K. C. Joseph, H. O. Jethva1, , Bhoomika Jogiya, C. K. Chauhan2 ,, A. D. B. Vaidya, M. J. Joshi1
Keywords
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- DOI 10.9790/3008-1204084753
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