In vitro multiplication of plantain cv. ‘INIVIT PV-2011’ (Musa AAB) in Temporary Immersion System
Journal Title: Biotecnología Vegetal - Year 2015, Vol 15, Issue 3
Abstract
The work was developed in the laboratory of Plant Biotechnology at Instituto de Investigaciones de Viandas Tropicales (INIVIT) with the aim of multiplying the plantain cultivar ‘INIVIT PV-2011’ (Musa AAB) in Temporary Immersion Systems (10.0 liters Nalgene bottles). The effect of immersion time (10, 20 and 30 minutes), the frequency of immersion (three, six and eight hours), the volume of culture medium per explant (20, 40, 60 and 80 ml), subculture time (15, 18, 21 and 25 days) and the inoculum density explants per bottle (20, 40, 60 and 80 explants / culture flask) were determined. The results allowed to establish an immersion time of 10 minutes with a frequency of immersion every three hours, 60 explants per bottle, 60 ml of culture medium and subculture explant at 18 days for multiplying this cultivar. A multiplication coefficient of 8.5 and plant material with suitable characteristics were obtained.
Authors and Affiliations
Milagros Basail Pérez, Victor Medero Vega, Yenisey Gutiérrez Sánchez, Marlenys Torres Delgado, Jorge López Torres, Arletys Santos Pino, Aymé Rayas Cabrera, Maricel Bauta Toledo, Yoel Beovidez García, Alexi Ortega Ortiz
Keywords
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