In vitro phosphate solubilization by Aspergillus and Penicillium strains and coffee plants growth promotion
Journal Title: Biotecnología Vegetal - Year 2019, Vol 19, Issue 1
Abstract
The availability of phosphorus in the soil for the growth of plants can be increased by the action of phosphate solubilizing microorganisms. The objective of this work was to determine the potential of Aspergillus and Penicillium strains, solubilizers of phosphates in vitro, to promote the growth of coffee plants obtained by somatic embryogenesis. In vitro tests were carried out with strains of Aspergillus and Penicillium grown in Pikosvkaya culture medium (PVK) to determine their solubilization capacity of phosphates. In addition, PVK and NBRIP liquid where the pH was measured were inoculated in culture medium. Two strains were selected and cultured for 72 h in PVK culture medium. Subsequently, suspensions of conidia in the culture filtrates were applied to the substrate (soil: compost) of Coffea canephora cv. 'Red Caturra' obtained by somatic embryogenesis. After 90 days of culture the height of the plants was measured (cm), the number of leaf pairs was quantified and the dry mass (g) of the biomass was determined. All strains tested showed translucent halos around the colonies in PVK culture medium indicative of phosphate solubilization and reduced pH in liquid culture media. The inoculation of Aspegillus sp. CCIBP-Asp 32 significantly increased the height of the plants and the biomass. The potential of these strains was demonstrated for the formulation of bioproducts that can be applied in green house or nursery to promote the growth of coffee plants in vitro obtained.
Authors and Affiliations
Mayra Acosta-Suárez, Mileidy Cruz-Martín, Yelenys Alvarado-Capó, Raul Barbón, Tatiana Pichardo, Eloísa Rodríguez, Alina Capote, Anabel Pérez
Keywords
Related Articles
- EP ID EP682889
- DOI -
- Views 259
- Downloads 1
Antioxidant enzymes preserving coffee quality in refrigerate environment
Post-harvest phases may cause biochemical and physiological changes with direct effects on coffee (Coffea sp.) grains quality during its storage. The aim of this study was to determine the relevance of antioxidant isoe...
Obtention of Moringa oleifera Lam. cv. ‘PKM criolla’ in vitro plants from nodal segments
Moringa oleifera L. is considered a perennial plant that has exceptional nutritional qualities. This species is very rich in protein, vitamins and minerals; that is why it is an important complement to the food and feed...
Genomic characterization by AFLP of Stevia rebaudiana Bertoni cultivated in the department of Antioquia (Colombia)
Stevia rebaudiana Bertoni, Asteraceae family (2n = 22), is a plant native from Paraguay, it has in leaves tissues a high production of some substances with strong edulcorant power, getting to be 320 sweet times but that...
Evaluation of phenotypic variability and early selection for rust of sugarcane (Puccinia melanocephala H. & P. Syd) in plants regenerated from irradiated calluses of the cane variety sugar ́SP 70-1284 ́
It was evaluated the variability fenotípic in early phases of selection from plants regenerated of the mutagenic treatment with dose of 30 Gy of radiations Gamma (source 60Co) applicated to calluses in growth of the dona...
In vitro propagation of Hylocereus purpusii Britton & Rose, a mexican species in danger of extinction
The purpose of this work was to evaluate different conditions and culture parameters for the in vitro establishment and multiplication of Hylocereus purpusii. Seeds were used as plant material and a workflow was develope...