In vitro propagation of a medicinal important plant Bacopa monnieri from nodal explants
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2017, Vol 5, Issue 1
Abstract
Bacopa monnieri, a traditional Indian medicinal plant with high commercial importance, is used as a neurotonic, immuno modulator, adaptogen transquilizing, memory and learning enhancing, cerebral activator, anti-ulcer, antispasmodic, anti-asthmatic ayurvedic herb. The study was undertaken in order to elaborate the tissue culture technique for Brahmi (Bacopa monnieri). In the present study nodal explants were taken for in vitro propagation. For sterilization, 0.01% HgCl2 was used as the sterilant. The explants gave best results when treated with the sterilant for 5 minutes. To induce bud-break, nodal segments were inoculated onto MS media supplemented with 1mg/l BAP. Basal MS medium without any plant growth hormone was used as control. Further multiplication was obtained on MS medium + BAP (1.0 mg/l) +IAA (0.5 mg/l). The shoots were rooted and best rooting was observed by IBA when incorporated in MS at different concentrations (0.1 - 0.3 mg/l) and 0.2 mg/l gave good results. After 30 days about 2.2 cm length was observed. Almost 70% of the rooted shoots survived during hardening.
Authors and Affiliations
Himisha Dixit, Ajay Thakur
Keywords
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