In Vitro Propagation of Date Palm Seedling Strain (Shaba) Grown Under Aswan Conditions
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 4
Abstract
The presentinvestigation is one of the first works on micropropagation of the seedling strain (Shabah) dry date palm (Phoenix dactylifera L.) grown at Aswan Governorate. Explants (shoot tips and leaf primordia) were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of casein hydrolysate (CH). The highest swelling and callus initiation percentage were achieved at 2 g/L of CH. Results indicated that the shoot tips gave the highest swelling and callus initiation percentage. The percentage of embryogenic callus formation obtained from shoot tip and leaf primordia explants reached the highest value, when cultured on MS medium with 3 mg/L 6-benzyl adenine (BA) + 10 mg/Lnaphthalene acetic acid (NAA) + 10 mg/L 2,4-dichlorphenoxy acetic acid (2,4-D), compared with other tested treatments. In addition, the same medium recorded the highest fresh weight of embryogenic callus. Addition of salicylic acid (SA) as a supplement to the MS medium led to a significant increase in number and percentage of germinated somatic embryos. The culture medium containing 50 mg/L SA gave the highest number and germination percentage of embryos for somatic embryogenesis derived from the shoot tip and leaf primordia explants. Polyethylene glycol (PEG) treatment at different concentrations significantly increased root number and reduced average root length by increasing PEG concentration as compared with plantlet produced in culture medium without PEG. MS medium supplemented with 4 g/L PEG resulted in the highest significant number of adventitious roots.Percentage of survival plantlets, which treatedwith PEG during rooting stage reached the highest value of survival percentage in acclimatization (80%) on MS medium supplemented with 10 g/L PEG.
Authors and Affiliations
Mohamed R. A. Abd- Alhady, Reda E. Abo Elfadl
Keywords
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- EP ID EP415129
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