In vitro propagation of Morus alba L. in semi-solid culture medium
Journal Title: Biotecnología Vegetal - Year 2005, Vol 5, Issue 2
Abstract
Apical buds as explants were used with the objective to propagate in vitro mulberry plants in semisolid MS culture medium suplemented with 6-BAP and KIN in their establishment and, with different combinations of 6- BAP with ANA in the multiplication phase. In vitro plants were evaluated during the acclimatization phase. It is necessary to supplement the basal MS culture media with 0.5 mg.l -1 of 6-BAP to induce the sprouting and, 0.5 mg.l -1 of 6-BAP and 0.5 mg.l -1 of ANA to multiply the mulberry by nodal segments. In the acclimatization phase a 95% of survival, 30.2 cm of height, 9.8 leaves and 2.02 g.plant -1 of dry mass was observed. In vitro propagation of mulberry was achieved as an alternative for plants production.
Authors and Affiliations
Enrique Salas Barbosa, Daniel Agramonte , Raúl Barbón , Felipe Jiménez , Raúl Collado, Martha Pérez, Odalys Gutiérrez
Keywords
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