In vitro propagation of the medicinal plant Morinda royoc L.
Journal Title: Biotecnología Vegetal - Year 2011, Vol 111, Issue 1
Abstract
Morinda royoc L., is a specie threatened by over collection due to its importance as medicinal plant. The in vitro propagation of M. royoc may help to solve the increasing demand of stock plants for field cultivation and also would contribute with in vitro conservation strategies and further breeding programs. The aim was to regenerate in vitro plants of M. royoc from shoot tips and nodal explants. Shoot cultures were established starting from tips and nodal segments from greenhouse grown plants. In the multiplication phase the highest multiplication rate was achieved when the culture medium was supplemented with 4.4 μM 6-benzylaminopurine (BA) and 2.9 μM indole-3-acetic acid (IAA). Addition of IAA to rooting medium influenced the quality of the plantlets and the survival rate after transplant to acclimatization stage. Survival 96.4% was achieved in plants cultured in the medium containing 2.9 μM IAA. The in vitro regeneration of M. royoc plants could be used either for the commercial clonal propagation of the species or for future studies on the production of secondary metabolites for pharmaceutical use given the medicinal properties of the species.
Authors and Affiliations
Elio Jiménez , Carlos Reyes , Pablo Machado , Naivy Pérez-Alonso , Alina Capote , Anabel Pérez , Bettina Eichler-Loebermann
Keywords
Related Articles
- EP ID EP683748
- DOI -
- Views 125
- Downloads 0
Sporophyte regeneration of Platycerium bifurcatum (Cav.) C.Chr. from in vitro germinated spores
In Cuba one of the most popular ferns, as an ornamental plant, is elkhorn (Platicerium bifurcatum) but its vegetative propagation is very slow so it is insufficient supply of this plant on the market. Besides, there is...
Identification and phylogeny of the tomato receptor-like proteins family
The receptor-like proteins (RLPs) play multiple roles in development and defense. In the current work 75 RLPs were identified in tomato (Solanum lycopersicum L.) using iterative BLAST searches and domain prediction. A p...
Influence of the time of year and type of bottle on embryogenesis somatic in papaya var. Red maradol
Somatic embryogenesis in papaya has been subjected to many researches. However, the effect of ex vitro and in vitro environment on this process has been little studied. The objectives of this work were to determine the...
Agronomic behavior of Gross Michel somaclones (AAA) resistant to Fusarium oxysporum var. cubense obtained using in vitro mutagenesis
The present work was realized in the Institute of Plant Biotechnology (IBP) with the objective to evaluate the agronomical behavior of several somaclons selected in the laboratory with possible resistance or tolerance to...
Damage to membrane and cellular respiration of Passalora fulva by extracts of leaves of Citrus aurantiifolia
The foliar mold of tomato (Solanum lycopersicum L.), produced by Passalora fulva, constitutes an important phytosanitary problem that affects the production of tomato in greenhouse. The use of plant extracts could cont...