In Vivo Anti-inflammatory Activity of Lipoic Acid Derivatives in Mice
Journal Title: Advances in Hygiene and Experimental Medicine - Year 2013, Vol 67, Issue 0
Abstract
Background: In mammals lipoic acid (LA) and its reduced form dihydrolipoic acid (DHLA) function as cofactors for multienzymatic complexes catalyzing the decarboxylation of α-ketoacids. Moreover, LA is used as a drug in a variety of diseases including inflammatory diseases. The aim of the study was to examine anti-inflammatory properties of LA metabolites.Material/methods:The present paper reports the chemical synthesis of 2,4-bismethylthio-butanoic acid (BMTBA) and tetranor-dihydrolipoic acid (tetranor-DHLA). BMTBA is one of the biotransformation products of LA, while tetranor-DHLA is an analogue of DHLA. Structural identity of these compounds was confirmed by 1H NMR. These compounds were assessed for their anti-inflammatory activity in mice. For this purpose, the zymosan-induced peritonitis and the carrageenan-induced hind paw edema animal models were applied.Results/conclusions: The obtained results indicated that the early vascular permeability measured at 30 min of zymosan-induced peritonitis was significantly inhibited in groups receiving BMTBA (10, 30, 50 mg/kg). The early infiltration of neutrophils measured at 4 hours of zymosan-induced peritonitis was inhibited in the group receiving BMTBA (50 mg/kg) and tetranor-DHLA (50 mg/kg). The results indicated that the increase in paw edema was significantly inhibited in the groups receiving BMTBA (50, 100 mg/kg) and tetranor-DHLA (30, 50 mg/kg). In summary, the present studies clearly demonstrated that both BMTBA and tetranor-DHLA were able to act as anti-inflammatory agents. This is the first study examining in vivo the anti-inflammatory properties of LA metabolites.
Authors and Affiliations
Brunon Kwiecień, Magdalena Dudek, Anna Bilska-Wilkosz, Joanna Knutelska, Marek Bednarski, Inga Kwiecień, Małgorzata Zygmunt, Maria Sokołowska-Jeżewicz, Jacek Sapa, Lidia Włodek
Keywords
Related Articles
- EP ID EP66914
- DOI -
- Views 144
- Downloads 0
Natural compounds involved in adipose tissue mass control in in vitro studies
The World Health Organization (WHO) has recognized obesity as an epidemic of the 21st century. Obesity is pathological fat accumulation in the body influenced by many factors: metabolic, endocrine, genetic, environmental...
Effectiveness and safety of probiotic preparations in clinical treatment of inflammatory bowel disease
Inflammatory bowel disease (IBD) is characterized by an aggressive immune response to luminal antigens including those of commensal microbiota, which are essential for intestinal homeostasis and appear to play a role in...
Role of tumor microenvironment in the formation and progression of skin melanoma
Melanoma develops from melanocytes localized mainly in skin, to a lesser extent in uvea and mucosal tissue. It is one of the most aggressive tumors, resistant to standard therapies. It is caused mainly by UV radiation. A...
Właściwości antynowotworowe galusanu epigallokatechiny zawartego w zielonej herbacie
Badania prowadzone in vitro i in vivo wskazują na możliwość wykorzystania katechin zielonej herbaty w chemioprewencji nowotworów. Najnowsze doniesienia naukowe mówią o hamującym wpływie galusanu epigallokatechiny na rozw...
HMGB1 – rola w progresji i terapii przeciwnowotworowej
Białko HMGB1 jest ewolucyjnie konserwatywnym białkiem o bardzo szerokim zakresie działania. Głównymi receptorami białka HMGB1 są receptory RAGE i TLR znajdujące się na komórkach układu odpornościowego i komórkach śródbło...