In Vivo Anti-inflammatory Activity of Lipoic Acid Derivatives in Mice
Journal Title: Advances in Hygiene and Experimental Medicine - Year 2013, Vol 67, Issue 0
Abstract
Background: In mammals lipoic acid (LA) and its reduced form dihydrolipoic acid (DHLA) function as cofactors for multienzymatic complexes catalyzing the decarboxylation of α-ketoacids. Moreover, LA is used as a drug in a variety of diseases including inflammatory diseases. The aim of the study was to examine anti-inflammatory properties of LA metabolites.Material/methods:The present paper reports the chemical synthesis of 2,4-bismethylthio-butanoic acid (BMTBA) and tetranor-dihydrolipoic acid (tetranor-DHLA). BMTBA is one of the biotransformation products of LA, while tetranor-DHLA is an analogue of DHLA. Structural identity of these compounds was confirmed by 1H NMR. These compounds were assessed for their anti-inflammatory activity in mice. For this purpose, the zymosan-induced peritonitis and the carrageenan-induced hind paw edema animal models were applied.Results/conclusions: The obtained results indicated that the early vascular permeability measured at 30 min of zymosan-induced peritonitis was significantly inhibited in groups receiving BMTBA (10, 30, 50 mg/kg). The early infiltration of neutrophils measured at 4 hours of zymosan-induced peritonitis was inhibited in the group receiving BMTBA (50 mg/kg) and tetranor-DHLA (50 mg/kg). The results indicated that the increase in paw edema was significantly inhibited in the groups receiving BMTBA (50, 100 mg/kg) and tetranor-DHLA (30, 50 mg/kg). In summary, the present studies clearly demonstrated that both BMTBA and tetranor-DHLA were able to act as anti-inflammatory agents. This is the first study examining in vivo the anti-inflammatory properties of LA metabolites.
Authors and Affiliations
Brunon Kwiecień, Magdalena Dudek, Anna Bilska-Wilkosz, Joanna Knutelska, Marek Bednarski, Inga Kwiecień, Małgorzata Zygmunt, Maria Sokołowska-Jeżewicz, Jacek Sapa, Lidia Włodek
Keywords
Related Articles
- EP ID EP66914
- DOI -
- Views 154
- Downloads 0
Nieswoiste mechanizmy odpornościowe w zakażeniach dolnego odcinka żeńskich narządów płciowych
Dolny odcinek żeńskich narządów płciowych jest miejscem kontaktu ze środowiskiem zewnętrznymi w przeciwieństwie do wyżej położonych struktur, nie jest sterylny. Istnieje wiele mechanizmów odpowiedzi nieswoistej, zapewnia...
Infection cases in infants and small children with atopic dermatitis – own observations
Introduction: The purpose of the work is to estimate the frequency of occurrence of various infections in infants and small children, aged between 0 and 36 months with atopic dermatitis in the practice of a family doctor...
Synergistic Interactions Between Anticancer Chemotherapeutics and Phenolic Compounds and Anticancer Synergy Between Polyphenols
Chemoprevention has recently gained a new dimension due to the possibility of studying the mechanisms of action of chemopreventive agents at the molecular level. Many compounds have been proved to inhibit early stages of...
Bakterie kwasu mlekowego i zdrowie: czy probiotyki są bezpieczne dla człowieka?
Wpływ Lactobacillus i Bifidobacterium na zdrowie człowieka jest badany już od wielu lat. Liczne badania in vivo i in vitro potwierdziły korzystne działanie wybranych LAB (bakterii kwasu mlekowego) w leczeniu i zapobiegan...
Zaawansowane produkty utleniania białek jako potencjalny czynnik diagnostyczny i prognostyczny w chorobach o wskazywanym udziale stresu oksydacyjnego
Możliwość diagnostycznego i/lub prognostycznego wykorzystania pomiaru stężenia zaawansowanych produktów utleniania białek (AOPPs) w chorobach przewlekłych o dobrze udokumentowanym udziale stresu oksydacyjnego (OS) w ich...