Influence of delay in blood-processing on the level of cell-free fetal DNA in maternal plasma sample
Journal Title: Di-er Junyi Daxue Xuebao - Year 2008, Vol 29, Issue 9
Abstract
Objective:To investigate the influencing factors of cell-free fetal DNA level in the maternal plasma during blood-processing.Methods: Aliquots of blood samples from pregnant women with male fetus were processed at 6 h and 36 h after sampling.The SRY and β-globin genes and the total DNA level were quantified by real-time quantitative PCR.Death of white blood cells was assayed by flow cytometry after stained with AnnexinⅤ/PI.The plasma DNase activity was assayed by radial enzyme-diffusion method and plasma lactate dehydrogenases(LDH) by rate method.Results: A 36 hour delay in blood-processing led to a significant increase in the total DNA and decrease in the fetal DNA (SRY gene) in the maternal plasma.The ratio of fetal DNA decreased from (10.3±5.6)% at 6 h after sampling to (3.0±2.1)% at 36 h after sampling under 4℃(P<0.05).No dead cells were identified in the blood sample 6 h after sampling; however,apoptosis and necrosis of white blood cells were identified 36 h after sampling.The activity of LDH at 36 h was significantly higher than that at 6 h (P<0.05).Radial enzyme-diffusion result showed that,though greatly decreased at 4℃,the DNase was still able to degrade DNA.Conclusion: Delay in blood-processing can lead to increase of the total free DNA in maternal plasma but decrease of fetal DNA,which might be related to the death of white blood cells and degradation of fetal DNA by plasma DNase,so the extraction of fetal DNA should be done as early as possible after sampling (within 6 h).
Authors and Affiliations
Qin LI
Keywords
Related Articles
- EP ID EP84208
- DOI -
- Views 139
- Downloads 0
LC-MS/MS in determination of telmisartan concentration in human plasma
表皮生长因子及其受体在多囊肾病大鼠体液及肾组织中的表达及意义
目的:观察常染色体显性多囊肾病大鼠体液及肾脏组织中表皮生长因子(EGF)及其受体(EGFR)的表达,探讨二者在多囊肾病发病过程中的作用。方法:采用ELISA方法检测多囊肾病Han:SPRD大鼠血液、尿液中EGF含量,蛋白质印迹法、原位杂交及免疫组织化...
Low-dose naloxone combined with sufentanil and ropivacaine for postoperative patient-controlled epidural analgesia in elderly patients undergoing total hip replacement
Objective:To evaluate the effect and safety of low-dose naloxone combined with sufentanil and ropivacaine for postoperative patient-controlled epidural analgesia (PCEA) in elderly patients undergoing total hip replacemen...
The development of quorum sensing phenomenon in pathogenic fungi
The quorum sensing commonly exists in procaryote kingdom, regulating various biologic functions. Recently similar phenomenon was also found in fungi world, which affecting both biofilm formation and dimorphism. In this a...
不同配比羟基磷灰石/胶原海绵复合物作为组织工程支架材料的比较
目的:选用不同配比羟基磷灰石和胶原海绵制备成复合材料，研究其结构和生物学特性，为构建组织工程人工胸壁支架材料提供实验依据。方法:按质量比11和12制备不同配比羟基磷灰石/胶原海绵复合材料，纯胶原海绵作为对照，进行扫描电镜检查...