Influence of the medium in bioautography-TLC screening test to the reliability of the results.
Journal Title: Annals of WULS, Forestry and Wood Technology; Zeszyty naukowe SGGW - Year 2015, Vol 92, Issue
Abstract
Influence of the medium in bioautography-TLC screening test to the reliability of the results. To the preliminary determination of the biological activity of selected chemicals used screening tests, allowing for quick identification of the fungistatic properties, such as bioautography. Because some factors (culture medium composition, tested microorganisms, extractive method, pH, solubility of the sample in the culture medium, etc.) can change results. The aim of the present work was to analyze the minimum inhibitory concentration (MIC) of selected fungicides (IPBC, 4,5-dichloro-2-octyl-2H-isothiazol-3-one, dichloro-2-n-octyl-4-isothazolin-3-one and 2-octyl-2H-isothiazol-3-one, N-alkyl(C12-18)-N,N-dimethyl-N-benzylammoniumchloride, chalcone) using various culture medium. This would allow to determine whether the change of the medium affects the results of screening test. The aim was also predicting the most preferred culture medium for screening - bioautography
Authors and Affiliations
PATRYCJA W. KWAŚNIEWSKA, ANNA K. PRZYBYŁ, Grzegorz Cofta
Keywords
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