Influence of transplantation of mesenchymal stem cells and interleukin-10 on experimental allergic encephalomyelitis course
Journal Title: Український неврологічний журнал - Year 2018, Vol 0, Issue 1
Abstract
Objective — to study the influence of the umbilical cordderived mesenchymal stem cells (MSC) and antiinflammatory IL10 injection on experimental allergic encephalomyelitis (ЕАЕ) course in rats. Methods and subjects. 50 white male rats with mean body weight 210 g were enrolled into the study. The recurrent course of the EAE was induced via injection of Freund’s adjuvant into the hind limb on the plantar surface. All animals were distributed into 4 groups depending on the MSC and IL10 treatment scheme. The severity of the EAE was defined via specific severity scale (sum of points). Statistical data was proceeded trough Microsoft Excel. Results. The severity of chronic recurrent EAE during its acme among all groups of animals was the moderate one (1.50 — 2.07 points). In group 1 (control group) we observed the acme of EAE on 22nd day. In group 2 we observed the insignificant worsening of the state of rats (with no statistical reliability) after the transplantation of MSC on the 17th day of EAE. The EAE acme drifted towards day 20, then we observed step-by-step cease of all clinical signs with complete recovery on 32nd day. The intravenous injection of IL10 in group 3 caused mild worsening of the state of rats, where in suboccipital injection of IL10 on the 17th day of EAE caused decrease of EAE severity within couple of days and complete cease of all clinical signs with complete recovery on 31st day. The EAE course before its acme in group 4 (twostage treatment with IL10 and MSC) was similar to group 3. The suboccipital injection of IL10 and MSC on the 17th day of EAE caused dramatic increase of EAE severity during first 2 days. Nevertheless, we observed fastened cease of all clinical signs of EAE in group 4 in comparison to group 2 and 3. Conclusions. The administration of antiinflammatory IL10 and MSC in their different combinations induced the complete cease of all clinical signs of EAE before 32th day of the experiment. The comparison of polynomial regressions proved higher efficacy of IL10 intravenous injections on the 10th day and IL10/MSC on the 17th day of the experiment.
Authors and Affiliations
L. D. Pichkur, S. А. Verbovska, V. V. Vaslovich, S. Т. Akinola, O. G. Deriabina, Ya. O. Pokholenko, O. K. Toporova, N. S. Shuvalova, V. А. Коrdium
Keywords
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