Intranasal Administration of Epidermal Neural Crest Stem Cells Improved Impairments in the Rat Model of Ischemic Stroke
Journal Title: The 2nd Annual Meeting of International Center for Neuroscience Research - Year 2021, Vol 2, Issue 1
Abstract
Stem cell-based therapy has received considerable attention as a potential candidate in the domain of ischemic stroke treatment. However, employing appropriate type of stem cells and effective delivery of these cells to the lesion site are still challenging which hinder the efficiency of stem cell-based therapy in this devastating condition. To take all into consideration, in this study we investigated the therapeutic effect of safe, noninvasive and braintargeted intranasal administration of epidermal neural crest stem cells (EPI-NCSCs) in the rat model of ischemic stroke. In doing so, EPI-NCSCs were obtained from bulge area of adult rat hair follicles and ischemic stroke was induced by middle cerebral artery occlusion (MCAO) for 30 minutes. Immediately after recovery from the anesthesia, EPI-NCSCs (2×106 cells/100µl phosphate buffered saline) were administrated through intranasal route in awake rats. Neurological function and body weight were evaluated before as well as 1 and 3 days after stroke. Infarct size was also measured by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining 3 days after the transplantation. Obtained results revealed intranasal administration of EPI-NCSCs improved functional outcome along with reduction of body weight loss. Furthermore, intranasal application of EPI-NCSCs reduced infarct size compared to the MCAO group. These data suggest the beneficial potential of EPI-NCSCs intranasal administration in the treatment of cerebral ischemia.
Authors and Affiliations
Seyedeh Maryam Mousavi*, Bijan Akbarpour, Afshin Borhani-Haghi, Mohammad Saied Salehi
Keywords
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