The Role of Tamoxifen (A Selective Estrogen Receptor Modulator) on Neurological Score, Blood-Brain Barrier and Brain Edema after Traumatic Brain Injury in Male Rats: The Role of Matrix Metalloproteinase-9.
Journal Title: The 2nd Annual Meeting of International Center for Neuroscience Research - Year 2021, Vol 2, Issue 1
Abstract
Introduction: Tamoxifen is an oral medication which is used for the treatment of breast cancer, and it acts on an estrogen receptor for agonist or antagonistic activity (effects) due to its effects on the environment. The metalloproteinase matrix is an enzyme that is produced by endothelial cells, microglia and astrocytes, and When the expression of the metalloproteinase-9 matrix will be progressed by an increased mucosal-permeability, it results in edema and nerve damage (the increased expression of the metalloproteinase-9 matrix results in an increased mucosal-permeability to edema and nerve damage). Therefore, in this study, we evaluated the effects of tamoxifen neuronal protection after induction of cerebral infarction in rats. Materials and methods: Wistar rats received different doses of tamoxifen (2.5, 5 and 10 mg / kg) intraperitoneally after induction of brain injury. Of course, animals were anesthetized and inserted into the chip before brain stroke induction. A brain stroke was made by Marmarou method and drug will be injected half an hour after the brain stroke. VCS of the animal was recorded at 0, 1, 4, and 24 hours after the traumatic brain injury. Beam Walk and Beam Balance tests were taken from an animal at this time (Beam Walk and Beam Balance). The level of permeability of the blood-brain barrier was elevated (monitored) by the Evans colored substance. After 24 hours, the animal is killed and its head is cut off, and the brain is fixed in nitrogen to be used for ELISA tests. Results: The results showed that brain damage reduced neurological scores, but tamoxifen 5mg / kg on the third day after trauma caused minimal difference with Sham or Intact groups (p <0.001). The brain edema and Evans blue content were significantly lower in the tamoxifen 5 mg / kg group than in the group receiving tamoxifen (2.5 mg / kg) (P <0.0001). Traversal time (BW) in the tamoxifen group 5 mg / kg did not have a significant difference with the Sham or Intact group on the second and third day after the trauma. In addition, tamoxifen injection (5 mg / kg) also reduces the secretion of metalloproteinase-9 matrix. Discussion: According to the findings of this study, it can be concluded that prescription of tamoxifen (5 mg / kg) in the time of impact in rats can reduce the consequences of brain trauma.
Authors and Affiliations
Mohaddese Shafiee Pour, Hamideh Tajik, Asal Safarbalou, Ali Siahposht-Khachaki*
Keywords
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