The Beneficial Potential of Human Dental Pulp Stem Cells and their Conditioned Medium in the Treatment of Rat Model of Ischemic Stroke
Journal Title: The 2nd Annual Meeting of International Center for Neuroscience Research - Year 2021, Vol 2, Issue 1
Abstract
Dental pulp stem cells (DPSCs) are considered as a promising type of stem cells for cell-based therapies. Hence, in this study we assessed the therapeutic effects of human (h)DPSCs and their conditioned medium (CM) in a rat model of ischemic stroke. Following 30 min middle cerebral artery occlusion (MCAO), hDPSCs (2×106 cells in 1 ml phosphate buffered saline) or their conditioned medium were administered into the carotid artery (hDPSCs-IA and hDPSCs-CM-IA groups). Furthermore, in other experimental groups, immediately after recovery from the anesthesia, hDPSCs (2×106 cells/100 µl phosphate buffered saline) or their conditioned medium were administrated through intranasal route in awake rats (hDPSCs-IN and hDPSCs-CM-IN groups). Before ischemia as well as 1 and 3 days after MCAO, neurological function test was performed. In addition, infarct volume were evaluated 3 days after cerebral ischemia. Our results revealed, the infarct volume significantly decreased in all four experimental groups compared to the MCAO group. Also, neurological function in hDPSCs and hDPSCs-CM transplanted groups (both intra-arterial and intranasal) were improved compared to the MCAO group.
Authors and Affiliations
Nahid Jashire Nezhad, Mohammad Nami, Mohammad Reza Namavar, Afshin BorhaniHaghighi, Mohammad Saied Salehi
Keywords
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