The Effects of Myricitrin on the Brain Edema, Neurological Scores and Blood-Brain Barrier Permeability, after Severe Traumatic Brain Injury in Male Rat: The Role of Matrix Metallopeptidase-9
Journal Title: The 2nd Annual Meeting of International Center for Neuroscience Research - Year 2021, Vol 2, Issue 1
Abstract
Introduction: Brain trauma is one of the leading causes of death among young people worldwide today and one of the leading causes of hospitalization. Myricitrin (Myr) is a natural compound, a flavonoid glycoside, which has neuroprotective properties of flavonoids, especially their antioxidant effects. Suggests that they may have therapeutic potential to eliminate neuronal death following neurotoxicity in brain tissue, but its exact mechanism is not yet known. Therefore, in this study, we investigated the neuroprotective effects of myricitrin after induction of brain injury in rats and investigated its mechanism. Methods: Thirty minutes after traumatic brain injury induction by Marmarou free fall method, Myricitrin three different doses (10, 20 and 40mg/kg) were administered intraperitoneally. VCS of animals were recorded perior (pre), after (D0), 24 hours later (D1), 48 hours (D2) and 72 hours (D3) after TBI induction. Vestibulomotor tests were evaluated by Beam Walk (BW) and Beam Balance (BB) tests in similar fashion. To determine permeability of BloodBrain Barrier (BBB) and brain edema 72 hours after TBI induction, Evans-Blue dye and Wet-Dry methods were employed respectively. Cerebrospinal fluid (CSF) was collected 72 hours after TBI induction to evaluate the levels of MMP-9. Results: Our findings showed that traumatic brain injury causes cerebral edema and destruction of the blood-brain barrier, changes in the neurological and equilibrium scores of the animal, and a significant increase in MMP-9 (P <0.001). 10 and 20 mg/kg Myr was able to eliminate these disorders compared to the control group, but at a dose of 20 mg these changes were more pronounced (P <0.0001). Myricitrin at doses of 20 and 10 mg / kg decreased MMP-9 after 72 hours, but 40 mg / kg did not have a significant effect on behavioral and biochemical values. Discussion: In view of the above considerations, it appears that myricitrin after induction of severe brain trauma at doses of 10 and 20 mg / kg has beneficial effects on severe traumatic brain injury by altering MMP-9 cerebrospinal fluid and improving blood-brain barrier. This effect was more pronounced at a dose of 20 mg / kg. Therefore, it has a dose-dependent neuroprotective effect on brain trauma, which requires more molecular and biochemical work to understand the mechanism of effects.
Authors and Affiliations
Mohaddese Shafiee Pour, Ali Siahposht-Khachaki*, Davood Farzin, Niki Pashaee Marandi, Asal Safar Balou
Keywords
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