Investigation of Compositions and Effects of Local Herbal Silybum marianum and Foeniculum vulgare extractions on Hospital Acquired Infections (HAI) and Cell Line of liver Cancer (HepG2) by MTT assays
Journal Title: Journal of Herbal Drugs - Year 2017, Vol 7, Issue 4
Abstract
Background & Aim: Increasing of drug resistances and more attention to the hazards of side effects of synthetic drugs has caused extensive researches on medicinal plant resources. The aim of this study was to determination of the chemical composition of native Silybum marianum and Foeniculum vulgare extractions in Ardabil, Iran; and investigation of their anticancer effect on liver cancer cell line HepG2 and antimicrobial effects on Staphylococcus aureus and Pseudomonas aeruginosa to propose a solution to control or treatment. Experimental: The chemical compositions of extractions performed through Soxhlet were analyzed by GC/MS system. The anticancer effect was assayed by MTT method and their antimicrobial activities were tested using disc-diffusion assay as well as MIC and MBC values estimated according to the microdilution method. Results: The analysis of S. marianum extract showed the presence of 29 compounds, mostly (2.63%) Nonane, (4.68%) Camphor, (2.84%) Borneol, (3.65%) Trans-anethole, (8.78%) Viridiflorol, (34.26%) α–Selinene, (2.34%) Longiborn-9-ene, (2.52%) amorphane-B, (5.00%) Erucylamide, (10.15%) o-Terphenyl and (46.8%) α-androst-8-ene; F. vulgare extract contained 14 compounds, mainly (5.53%) Fenchone, (5.53%) α- Thujone, (2.84%) Nonane, (79.10%) trans-Anethol and (2.51%) Hentriacontane. The effects of F. vulgare extract on Staphylococcus and S. marianum extract on Pseudomonas were remarkable. In the combined design, there was no clear effect. MIC and MBC values confirmed the results of the sensitivity test. The cytotoxic effects of extractions on liver cancer cells were the optimum in 25μg/ml after 48 hours. Recommended applications/industries: According to the results, these compounds are able to replace by chemical preservatives in food and drugs industries.
Authors and Affiliations
Golsoom Samadi Andzagi; hashem yaghoubi; Masoud Fardin
Keywords
Related Articles
- EP ID EP23533
- DOI -
- Views 272
- Downloads 16
Folk Herbal Veterinary Medicines of Tehran Watershed (Iran)
Background & Aim: The use of medicinal plants is an option for livestock farmers who do not want to use allopathic drugs under certified organic programs or cannot afford to use allopathic drugs for minor health probl...
Antimicrobial Effects of Zataria multiflora Essential Oils on Acinetobacter Strains Isolated from Clinical Specimens
Background & Aim: The aim of this study was to investigate the antimicrobial effects of Zataria multiflora against some Acinetobacter baumannii strains isolated from clinical samples. Experimental: Twelve strains of A...
Effect of different level water and nitrogen on morphological and physiological traits of Matricaria aurea L.
Background & Aim: Matricaria aure is one of medicinal plants belonging to Asteraceae family. They naturally grow in some regions of the country and have different medical properties and various applications in medicine p...
The study of flora Ghaysari region in Chaharmahal va Bakhtiari province with 179 species of medicinal plants
Background & Aim: The present study was to identify the medicinal plant species and introduced as one of the key priority areas for conservation and determination of endangered species were done. Experimental: The metho...
Effects of aluminosilicate zeolite application on morphological traits and chemical compositions of Aloe (Aloe vera L.) under drought stress
Background & Aim: Aloe vera is used in the food and pharmaceutical as an aid to effective treatment. The purpose of this study is to improve growth, increase production of valuable metabolites and increase the sugars in...