Investigation of leptin polymorphism by PCR-RFLP in Iranian buffalo
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2012, Vol 3, Issue 8
Abstract
Leptin is a 16 KD protein that synthesis by white adipose tissue and it involves in regulation of feed Intake, energy balance, fertility and immune function. In cattle, the leptin gene is located on chromosome four. It consists out of 3 exons and 2 introns of which only 2 exon are translated in to protein. We used the polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) technique to screen for DNA polymorphisms of the leptin gene in 70 buffaloes in Shadegan, Ahwaz, Dashte azadegan, Dezfoul and Shoshtar cites (khuzestan provice). In all sample, we amplified a 422 fragment consisting of part of entron 2. The amplified fragment digested with sau3A restriction endonuclease and subjected to electrophoretic separation in ethidium bromid-strained 2% agarose gel. After enzymatic digestion of the PCR products by sau3A enzyme generated a 303, 88 and 32 bp fragment of DNA that indicating BB genotype of leptin in buffalo.
Authors and Affiliations
Rahimnahal. Somayeh*| Department of Animal Science Ramin (Khuzestan Agriculture and Natural Resources University Ramin, Mollasani, Khuzestan Iran),s.rahimnahal@yahoo.com, Keshavarz. Vida| Department of Animal Science Ramin (Khuzestan Agriculture and Natural Resources University Ramin, Mollasani, Khuzestan Iran), Sadr. A| Department of Animal Science Ramin (Khuzestan Agriculture and Natural Resources University Ramin, Mollasani, Khuzestan Iran), Moosavi. Mona| Department of Animal Science Ramin (Khuzestan Agriculture and Natural Resources University Ramin, Mollasani, Khuzestan Iran)
Keywords
Related Articles
- EP ID EP5092
- DOI -
- Views 419
- Downloads 23
The contribution of Quran in the development of human being's society
Islam commands that one's environment must be cared for,protected and forbids destructing it in any way. It does so through the following means:It teaches us to establish justice. It specifies roles for menand women, whe...
The Preferable Presentation Method of Models of the Cash Flow Statement
In most countries, the statement of cash flows contains three sections; though, it has five sections in Iran. Also, for the first time, we have presented four-way classification of the cash flow statement along with the...
Comparison of Derived Instantaneous Unit Hydrograph fromH2U, Laplaceand H2U-Nash, Case Study:North Karoon River Basin, Iran
Flooding is a kind of natural disaster affects life, livelihoods and human assert sat an unexpected scale. Recently there is heightened research interest in simulation of flood hydro graph as watershed response to rainfa...
Examining the Effect of Abacus Training On Creativity and Self-Efficacy of Fifth-Grade Female Students in Urmia City
The aim of the present study is to examine the effect of abacus training on creativity and self-efficacy of fifth-grade female students in Urmia. The study is applied and quasi-experimental in terms pf he goal and contro...
A Study of Relationship between Accruals over Life Cyclesof Food and Drug Companies Listed in Tehran Stock Exchange
Accruals are among important variables often used in accounting studies as a proxy for quality of information. Recently, a number of studies have examined the effects of lifecycle stages on accruals. The present study wa...