INVESTIGATION ON ANTIMICROBIAL ACTIVITY OF BIOSURFACTANT PRODUCED BY PSEUDOMONAS FLUORESCENS ISOLATED FROM MANGROVE ECOSYSTEM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 1
Abstract
The aim of this present study is to investigate the antimicrobial activity of rhamnolipid biosurfactant produced by Pseudomonas fluorescens MFS03 isolated from mangrove forest soil using groundnut oil cake as substrate. The biosurfactant was extracted with an equal amount of ethyl acetate and the concentrated extract was subjected to FT-IR analysis. The important adsorption bands at 3466.24, 2926.45, 1743.47, 1407.30 and 1162.26 cm-1indicate the chemical structure of rhamnolipid. The rhamnolipid biosurfactant was investigated for the potential antimicrobial activity by using disc-diffusion method against Gram positive bacteria (Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Methicillin resistance S. aureus) Gram negative bacteria (Escherichia coli, Salmonella typhimurium) and a yeast (Candida albicans). The biosurfactant showed distinct antibacterial activity towards tested bacteria and shows an antifungal activity against yeast. The biosurfactant with different concentration was performed for the evaluation of antimicrobial activity. Maximum antimicrobial activity of the biosurfactant (50µl) was observed in S. aureus (23 mm) and it was found that the biosurfactant activity was dependent on the concentration. So it could be used as a therapeutic agent in pharmaceutical application.
Authors and Affiliations
M Govindammal , R Parthasarathi
Keywords
Related Articles
- EP ID EP120072
- DOI -
- Views 109
- Downloads 0
CRY 1Ac AND CRY 2Ab EXPRESSION IN TRANSGENIC BT COTTON HYBRIDS AS INFLUENCED BY NUTRITION AND SPACING IN RAINFED VERTISOLS
Cotton is one of the major commercial crops in Andhra Pradesh and cultivation of Bacillus thuringiensis cotton played a major role in the current scenario. Tulasi (9) BG II, Mallika BG II, Bunny BG II are popular Bacillu...
DIURETIC AND ANTI-INFLAMMATORY ACTIVITY OF AQUEOUS EXTRACT OF AERVA SANGUINOLENTA (L.) BLUME.
The study was designed to evaluate the diuretic and anti-inflammatory potency of aqueous extract of whole plant of Aerva sanguinolenta in wistar albino rats. Different parameters viz. total urine volume, urine concentrat...
ETHNOBOTANY, PHYTOCHEMISTRY AND PHARMACOLOGY OF MELOCHIA CORCHORIFOLIA L.
Plant profile, vernacular names, folk medicinal uses, ethno botany, phytochemistry and pharmacology of an important medicinal plant Melochia corchorifolia L. belonging to the family Sterculiaceae are reviewed in this pap...
DEVELOPMENT OF UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF COLCHICINE IN PHOSPHATE BUFFER SALINE pH 6.4
The present investigation explores a method to analyze colchicine in phosphate buffer saline pH 6.4. Based on the spectrophotometric characteristics of colchicine, λmax selected was 353.8 nm. Analytical parameters valid...
GC-MS ANALYSIS OF ETHANOLIC EXTRACT OF POLYPODIUM DECUMANUM
A gas chromatography-mass spectrometry was carried out on a Agilent GC-MS 5975 under electron impact ionization (70 eV). The chromatographic column for the analysis was done by the HP5MS capillary column (30 m x 0.25 mm...