INVITRO ANTIOXIDANT ACTIVITY AND HPTLC ANALYSIS OF WITHANIA SOMNIFERA
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 6
Abstract
Withania somnifera is a well known medicinal plant having wide range of medicinal properties. HAEWS, MEWS and AEWS were comparatively standardized for the content of Withanolide D by HPTLC. All the samples were screened for the possible in-vitro antioxidant activity for 1,1-diphenyl-2-picryl-hydrazil (DPPH.) free radical scavenging, hydrogen peroxide scavenging, flavonoid content, polyphenolic contents, and lipid peroxidation effects. The Withanolide D content of HAEWS, MEWS and AEWS was found to be 0.103, 0.067, and 0.036% (w/w) respectively. The Polyphenol and flavonoid content of HAEWS was found to be 35.51, 17.68 (w/w) respectively. All the tested samples showed marked antioxidant activity in which HAEWS showed prominent activity. HAEWS showed significant (p<0.01) (DPPH) free radical scavenging (67.38%), hydrogen peroxide scavenging (61.05%) and lipid peroxidation (63.86%) , reducing power (0.93%) at the concentration of 1000μg/ml. These various antioxidant activities were compared to standard antioxidant such as ascorbic acid.
Authors and Affiliations
Makhija Manju*| M.Pharm, Rajasthan University of Health Science, Kumbha Marg , Pratapnagar, Jaipur, Rajasthan, India, Gupta Maheshkumar K| Ph.D, Principal, Kota college of Pharmacy, Kota,Rajasthan,India.
Seroprevalence of Herpes Simplex Virus (HSV-1 and HSV-2) Infections in Sudanese Renal Transplant Recipients
Introduction: Viruses are among the most common causes of opportunistic infections after transplantation, because of their immunosupressed condition. Aim: This study aimed to determine Herpes Simplex Virus-1 and Herpes...
PLATELET COUNT, PLATELET INDICES, ERYTHROCYTE SEDIMENTATION RATE AND C-REACTIVE PROTEIN IN PULMONARY TUBERCULOSIS PATIENTS IN ABU-ANJA HOSPITAL
Background: Tuberculosis (TB) is a major public health problem in Sudan. The pulmonary type is the most important form of tuberculosis, continues to be one of the most wide spread infectious diseases. The differential di...
CARBAZOLE: IT’S BIOLOGICAL ACTIVITY
Carbazole alkaloids constitute an important class of naturally occurring heterocycles, isolated from the Rutaceaefamily. First Carbazole alkaloids were isolated as natural products from Murraya koenigii that exhibited s...
PHENYTOIN INDUCED TOXIC EPIDERMAL NECROLYSIS; A CASE REPORT
Several drugs are at high risk of causing Toxic Epidermal Necrolysis (TEN) including Anti-convulsants such as phenytoin, carbamazepine and phenobarbital. Other drugs include antimicrobials like sulfonamides followed by N...
A Validated HPLC Method for the Determination of Rabeprazole in Bulk and Pharmaceutical Dosage form.
A reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for the estimation of Rabeprazole in bulk and tablets dosage forms. The separation was achieved on C18 analytical colum...