Isolation and Characterisation on Proteus vulgaris from Mangrove Sediment of Vellar Estuary for Urease Production
Journal Title: Journal of Biological and chemical Chronicles - Year 2018, Vol 4, Issue 2
Abstract
Urease is a virulence factor found in various pathogenic bacteria. It is essential in colonization of a host organism and in maintenance of bacterial cells in tissues. Due to its enzymatic activity, urease has a toxic effect on human cells. The present study focused on the production of urease by a Proteus vulgaris isolated from a sediment sample of Vellar estuary, Porto Novo, Tamil Nadu, India. Secondary screening for quantitative urease estimation was done by phenol-hypochlorite assay. Based on the maximum enzyme activity on urease slant and plate assay as well as quantitative biochemical enzyme assay, most potential strains were chosen for further studies. An excellent correlation was found between test and standard urease which evidenced the possibilities of its industrial applications as urea diagnosis and reduction. Proteus vulgaris is an ideal product for the industrial production and various applications like determining and reducing urea contaminants.
Authors and Affiliations
A. Rebekkah Shanthakumari, M. Boominathan
Keywords
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