ISOLATION AND CHARACTERIZATION OF A FLAVONOID FROM ETHANOLIC EXTRACT OF ALTERNANTHERA SESSILIS LINN.
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2017, Vol 6, Issue 1
Abstract
This study was performed to isolate and characterize the flavonoid present in Alternanthera sessilis Linn. Direct soxhlet extraction process was adopted for extraction by using 95% ethanol and vacuum evaporator was used for drying the extract. The concentrated ethanolic fraction was subjected to thin layer chromatography and column chromatography for isolation. The isolated compound was identified as a flavonoid by confirming the standard flavonoid tests: viz. Shinoda’s test. The Rf value of isolated flavonoid was calculate and different physical tests were also performed to find out its physical characteristics. Characterization of isolated flavonoid was done by FTIR, 1H NMR and MASS. The IR spectrum indicated the presence of hydroxyl and carbonyl functions. 13C NMR signal indicated the presence of hydroxyl group and unsaturated keto function and four methoxy group at different position of flavones skeleton. The 1H-NMR further showed the presence of three hydroxyl, four methoxy groups and three methine groups. On the basis of chemical and spectral analysis the structure was elucidated as 3´, 3, 6, 7-tetramethoxy - 4´, 5, 8-trihydroxy flavones.
Authors and Affiliations
Mrinmay Das*| PRIST University, Vallam, Thanjavur, Tamilnadu-613403, Ashok Kumar D| Department of Pharmacy, Pratistha Institute of Pharmaceutical Sciences, Suryapet Dist, Andhra Pradesh-508214., Jyotirmoy Deb| Department of Pharmacy, S. Chaavan College of Pharmacy, Jangalakandriga (Vi), Nellore Dist, Andhra Pradesh-524346., Durga Srinivasa Rao| Department of Pharmacy, S. Chaavan College of Pharmacy, Jangalakandriga (Vi), Nellore Dist, Andhra Pradesh-524346.
Keywords
Related Articles
- EP ID EP3811
- DOI -
- Views 359
- Downloads 20
A CASE OF PATHOLOGICAL FRACTURE OF SHAFT OF FEMUR IN HYPERPARATHYROIDISM - TREATMENT WITH TITANIUM ELASTIC NAILING
Primary hyperparathyroidism is associated with a significant increase in the risk of pathological fractures. Persistent osteopenia secondary to increased calcium mobilisation from bones makes these patients highly suscep...
CHARACTERIZATION, OPTIMIZATION AND FORMULATION OF NIOSOME CONTAINING NAPROXEN
Niosomes are vesicular delivery systems which can be formed by aqueous dispersion of non-ionic surfactant films. An effort was made to formulate the Naproxen Niosomes and incorporate the Niosomes into the gel. Formulatio...
THE ANTIMICROBIAL EFFECT OF AQUEOUS EXTRACT OF TAMARIND (TAMARINDUS INDICA) LEAVES
This study is comparative-experimental work. It was conducted in National Center for Research (Sudan) during the period from 28th of August to 20th December 2013 to investigate the antimicrobial activity of aqueous extra...
Severe periodontitis associated with Marfan syndrome- an existence or a co-existence.
Marfan syndrome is the most common dominant autosomal genetic disorder of connective tissue origin. Over the years various diagnostic criteria have been developed for this entity, the earliest among them is the Berlin No...
PHYTOCHEMICAL AND ANTIMICROBIAL ANALYSES OF ESSENTIAL OILS OF FAGONIA CRETICA L.
Fagonia cretica L.belongs to Zygophyllaceae is having very important and significant Phytocemicals. The entire plant of Fagonia cretica L.was shade dried, and the powder was subjected to hydrodistillation to get essentia...