Isolation and characterization of stigmast-5-en-3β-ol (β-sitosterol) from the leaves of Hygrophila spinosa T. Anders
Journal Title: International Journal of Pharma Sciences and Research - Year 2010, Vol 1, Issue 2
Abstract
Purpose: [i]Hygrophila spinosa[/i] T. Anders (Acanthaceae) commonly known as ‘Talmakhana’ in Hindi contains a number of phytoconstituents viz. alkaloids, phytosterols, glycosides, amino acids, proteins, phenolic acids, enzymes, vitamins, sugars, minerals, flavonoids, gums & mucilage, terpenoids etc. The objective of the present study was to isolate and characterize phytoconstituents(s) from the chloroform extract of [i]Hygrophila spinosa[/i] leaves. Methods: Chloroform extract was subjected to column chromatography and eluted with solvent mixtures of increasing polarity, composed of petroleum ether, benzene and chloroform to isolate phytoconstituents. The structure of the isolated compound was established on the basis of elemental analysis and spectroscopic evidences (IR, UV, 1HNMR, 13CNMR, MS). Results: A sterol, stigmast-5-en-3β-ol was isolated from the chloroform extract of the leaves of the plant. The yield of the compound was 0.0046% w/w, m.p. 136-1380C, λ[sub]max[/sub] in EtOH: 206 nm, Rf value 0.72 in Toluene: Ether: Cyclohexane (5:2:1). Conclusions: [i]Hygrophila spinosa[/i] contains β-sitosterol which may be responsible for various pharmacological activities of the plant.
Authors and Affiliations
Arjun Patra , S. Jha , P. N. Murthy , Manik , A. Sharone
Keywords
Related Articles
- EP ID EP97138
- DOI -
- Views 129
- Downloads 0
Does screening of pregnant women prevent mother to child transmission of HIV?A study in nsukka urban area of Enugu State, Nigeria.
[b]Background:[/b] One of our best opportunities for progress against AIDS lies in preventing mothers from passing on the HIV virus to their children. Worldwide, close to 2,000 babies are infected with HIV every day, dur...
Introducing Integrated Teaching in Undergraduate Medical Curriculum
The medical curriculum is vast and students are expected to learn many subjects at the same time. The teachers are also involved in a number of activities apart from teaching like research , administrative, updating thei...
SPECTROPHOTHOMETRIC DETERMINATION OF GATIFLOXACIN THROUGH COMPLEXATION WITH SURFACTANT
A simple, quick and sensitive U.V spectrometric method has been developed for the quantitative estimation of Gatifloxacin in bulk and pharmaceutical formulation. The method was based on the complexation with neutral surf...
Determination of Alprazolam in Rabbit Plasma by GC-MS Method
This paper describes a gas chromatography-mass spectrometry (GC-MS) method for determination of alprazolam in rabbit plasma. Alprazolam and internal standard (IS) medazepam were extracted from plasma by using liquid-liqu...
Method Development and Validation for The Simultaneous Estimation of Ofloxacin and Ornidazole in Tablet Dosage Form by Rp-Hplc
A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of Ofloxacin and Ornidazole in combination. The separation was carried out using a mobile...