Isolation and characterization of stigmast-5-en-3β-ol (β-sitosterol) from the leaves of Hygrophila spinosa T. Anders
Journal Title: International Journal of Pharma Sciences and Research - Year 2010, Vol 1, Issue 2
Abstract
Purpose: [i]Hygrophila spinosa[/i] T. Anders (Acanthaceae) commonly known as ‘Talmakhana’ in Hindi contains a number of phytoconstituents viz. alkaloids, phytosterols, glycosides, amino acids, proteins, phenolic acids, enzymes, vitamins, sugars, minerals, flavonoids, gums & mucilage, terpenoids etc. The objective of the present study was to isolate and characterize phytoconstituents(s) from the chloroform extract of [i]Hygrophila spinosa[/i] leaves. Methods: Chloroform extract was subjected to column chromatography and eluted with solvent mixtures of increasing polarity, composed of petroleum ether, benzene and chloroform to isolate phytoconstituents. The structure of the isolated compound was established on the basis of elemental analysis and spectroscopic evidences (IR, UV, 1HNMR, 13CNMR, MS). Results: A sterol, stigmast-5-en-3β-ol was isolated from the chloroform extract of the leaves of the plant. The yield of the compound was 0.0046% w/w, m.p. 136-1380C, λ[sub]max[/sub] in EtOH: 206 nm, Rf value 0.72 in Toluene: Ether: Cyclohexane (5:2:1). Conclusions: [i]Hygrophila spinosa[/i] contains β-sitosterol which may be responsible for various pharmacological activities of the plant.
Authors and Affiliations
Arjun Patra , S. Jha , P. N. Murthy , Manik , A. Sharone
Keywords
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