SIMPLE COLORIMETRIC ASSAY FOR MICROGRAM DETERMINATION OF BROMHEXINE HYDROCHLORIDE WITH MBTH AND 2, 2’ BIPYRIDYL
Journal Title: International Journal of Pharma Sciences and Research - Year 2010, Vol 1, Issue 2
Abstract
The present work describes two new spectrophotometric methods for the determination of bromhexine hydrochloride in bulk and formulations. Method A is based on the reduction of Fe3+ into Fe2+ by the drug followed by the complex formation of Fe2+ with 2, 2’ bipyridyl to form cherry red colored chromogen having maximum absorbance at 510nm and Method B is based on oxidation of 3-methylbenzothiazolinone-2-hydrazone (MBTH) by FeCl3 followed by its coupling with the drug in acidic medium forming an intense green colored chromogen with absorbance maxima at 630nm. Beer’s law is obeyed in the range of 5-25 μg/ml with molar absorptivity of 1.113 x 104 for method A and 2-10 μg/ml with molar absorptivity of 1.128 x 104 for method B. The results were favorably compared with those obtained by reference UV spectrophotometric method. The accuracy, precision, and the linearity ranges of the methods have been determined, and they have been validated by analyzing the title drug. No interference was observed from common pharmaceutical adjuvants. These two methods were successfully applied to the pharmaceutical formulations, capsules, tablets.
Authors and Affiliations
G. Vijaya Raja , G. Venu gopal , V. Mounika , S. Satyavathi , Ch. Lavanya
Keywords
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SIMPLE COLORIMETRIC ASSAY FOR MICROGRAM DETERMINATION OF BROMHEXINE HYDROCHLORIDE WITH MBTH AND 2, 2’ BIPYRIDYL
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