Isolation and Identification of Bacterial Culture for Azo Dye Degrading Capability
Journal Title: International Journal of Research in Chemistry and Environment (IJRCE) - Year 2012, Vol 2, Issue 4
Abstract
Synthetic dyes are extensively used in textile dyeing, paper, printing, color, photography, pharmaceutics, cosmetics and other industries. Among these, azo dyes represent the largest and most versatile class of synthetic dyes. Approximately 10-15% of the dyes are released into the environment during manufacture and usage. Traditional methods of treatment are found to be expensive and have operational problems. In the present studies bacterial cultures were isolated from Environmental Treatment Plant Naroda G.I.D.C., Ahmedabad, Gujarat, India, were subjected for acclimatization to C.I. Reactive Blue 250 (RB 250), an azo dye in the Bushnell and Haas Broth (BHB). The most promising bacterial isolate was used for further dye degradation studies. The strain showed complete decolorization of the selected dye (RB 250- 100 mg/L) within 8 hr in static condition. The optimum pH, temperature, inoculum size and carbon and nitrogen sources for the decolorization was studied at 7.0, 37°C, glucose (0.2 %) and nitrogen (0.5 %) respectively. The biodegradation was monitored by UV-Vis, HPTLC and FTIR analysis. Down Flow Fixed Film Batch Bioreactor was studied, gave good response up to 200 mg/L RB 250 dye concentration. The results suggest that the isolated organism SpNB6 as a useful tool to treat waste water containing reactive dyes.
Authors and Affiliations
Murty D. , Patel D. , Soni Rakesh, Bhatt Nikhil
Keywords
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