Isolation and Screening of Cellulase Producing Thermophilic Bacteria from Compost Piles and Optimization of Cellulase Production
Journal Title: International Journal of Advanced Biotechnology and Research. - Year 2016, Vol 7, Issue 1
Abstract
BSTRACT The cellulase producing bacteria were isolated from compost pile collected from Navagam, District- Navsari, Gujarat, India. Total 5 isolates were obtained by the primary screening technique from which 2 isolates were showing maximum cellulase activity. These 2 isolates were then evaluated by secondary screening for enzyme production. These 2 isolates T1 and T2 were selected as most efficient enzyme producers and their specific enzyme activity in the crude sample was found to be 0.52 U/mg and 0.59 U/mg and of partially purified sample was found to be 0.20 U/mg and 0.18 U/mg respectively. Isolates were tentatively characterized on the basis of their morphological, cultural and biochemical characteristics, T1 and T2 both were identified to be Bacillus species. Further partial purification of the cellulase enzyme was carried out by ammonium sulphate precipitation followed by dialysis. Optimization of different parameters was carried out for the production of cellulase by both efficient isolates T1 and T2.
Authors and Affiliations
Trusha B. Mistry, Nilofar M. Shaikh, Hetal N. Rana and Nimisha D. Patel
Keywords
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