ISOLATION OF AN α-GALACTOSIDASE FROM BIFIDOBACTERIUM LONGUM ЛМ-6 AND IDENTIFICATION OF FUNCTIONAL GROUPS OF THE CATALYTIC CENTER OF ENZYME
Journal Title: Мікробіологія і біотехнологія - Year 2016, Vol 0, Issue 1
Abstract
Aim. Receiving enzyme of an α-galactosidase of Bifidobacterium longum LM-6. Identification of the functional groups of the catalytic site of the enzyme. Methods. Cultivation and accumulation of biomass of bifidobacteria. Identification of functional groups of the catalytic center of hydrolytic enzyme. Results. On acellular extract of enzyme of an α-galactosidase has been received, based on research and also activity of the studied enzyme is defined. The purification of the enzyme by means of the various organic solvents is carried out. The functional groups of the catalytic center of an α-galactosidase Bifidobacterium longum ЛМ-6 have been identified. It is established that at the active center of an α-galactosidase Bifidobacterium longum ЛМ-6 there are carboxyl and imidazolny groups of proteins, which take part in hydrolysis of galaktooligosakharid. Conclusions. The functional groups of the catalytic site of an α-galactosidase Bifidobacterium longum ЛМ-6 are identified for the first time. It is established that at the active center of an α-galactosidase Bifidobacterium longum ЛМ-6 there are carboxyl and imidazolny groups of proteins.
Authors and Affiliations
A. P. Petrosyants
Keywords
Related Articles
- EP ID EP318922
- DOI 10.18524/2307-4663.2016.1(33).65377
- Views 89
- Downloads 0
MUCOADHESIVE GEL WITH IMMOBILIZED LYSOZYME AND QUERCETIN
Aim. To develop mucoadhesive gel with jointly immobilized lysozyme and quercetin and to investigate biochemical, physical, physico-chemical and biological properties of the complex preparation. Methods. The bacteriolytic...
INFLUENCE OF QUATERNARY SALTS OF AMMONYUM ON GROWTH OF SULPHATE-REDUCING BACTERIA
Aim. To compare the growth of the monocultures of suphfate-reducing bacteria of Desulfomicrobium and Desulfovibrio genera under conditions of corrosion inhibition by biocide inhibitors – quaternary ammonium salts. Method...
PIGMENTS OF RHODOPSEUDOMONAS YAVOROVII IMV B-7620
Aim. Determination of spectra of photosynthetic pigments of the purple nonsulfur bacteria R. yavorovii IMV В-7620. Methods. Bacteria were grown in liquid modified medium of ATCC No. 1449 and tryptic soy agar under anaero...
THE EFFICIENCY OF USING ALGA EXTRACTS IN THE MICROCLONAL REPRODUCTION OF SOME PLANT SPECIES
Aim: to study the development of explants of some cultivated plants in vitro conditions using MC medium with addition of algae extracts. Materials and methods: the standard Murashige and Skoog nutrient medium (1962) with...
ACTIVITY AND KINETIC PROPERTIES OF ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE IN THE INTESTINAL SULFATE-REDUCING BACTERIA
Adenosine 5′-phosphosulfate (APS) reductase activity and the kinetic properties of the enzyme from intestinal sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 has never been well-charact...