Isolation, Optimization and Characterization of ?-Amylase from Bacillus Alcalophilus
Journal Title: International Journal of Science and Research (IJSR) - Year 2013, Vol 2, Issue 7
Abstract
Amylase is one of the most important enzymes widely used in paper, textile, pharmaceutical industries etc. In the present investigation a thermostable, alkalophilic amylase was isolated from underlying soil samples of domestic wastes, and was screened on starch agar medium. The amylase activity was measured using colorimetric method at 540nm. Among forty five isolates, thirteen demonstrated clear zone of hydrolysis around the colonies when flooded with Gram
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