L-Arginine optimization medium for hairy root induction of madder (Rubia tinctorum L.) based on one factor model of response surface methodology
Journal Title: Scientific Journal of Biological Sciences - Year 2014, Vol 3, Issue 12
Abstract
Medium optimization for hairy root cultures producing secondary metabolites was studied through statistical experimental design. In the following, one factor model of response surface methodology (RSM) was employed to formulate the L-Arginine amino acid levels alongside three categorical factors including bacterial strains (ATCC 15834, C58C1 and R1000), type of explant (Leaf and Stem) in co-cultivation media (B5 and MS) for hairy root induction of Madder (Rubia tinctorum L.). Design of experiment and data analysis was carried out by using Expert-Design® 7.1 software. According to the results, modeling and optimization conditions, including L-Arginine concentration 1.00 mM; bacterial strain; C58C1, leaf explant and B5 medium for HR induction frequency equal to 58% was evaluated (Desirability point=0.986). These optimal conditions predicted by RSM were confirmed to enhance hairy root induction as an application potential for biotechnological implementation to produce the anticipated compounds.
Authors and Affiliations
M. Ghorbani*| Master Degree of Agricultural Biotechnology, Agronomy and Plant Breeding Department, University of Tehran, IRAN., M. Omidi| Professor of Molecular Genetics and Biotechnology, Department of Agronomy and Plant Breeding, University of Tehran, IRAN., S. A. Peighambari| Professor in Plant Breeding, Department of Agronomy and Plant Breeding, University of Tehran, IRAN.
Reproductive biology of big-eye grunt Brachydeuterus Auritus in Ivory coast fishery (West Africa)
Reproductive biology of Big-eye grunt, Brachydeuterus auritus resulting from Ivory Coast marine fishery were studied by monthly sampling from August 2013 to July 2014. Samplings were carried out along the Ivorian littora...
Morphometric study of some Salvia L. (Lamiaceae) species in Iran
This study concerns the morphological studies in some Salvia L. (Lamiaceae) species in Iran. In order to elucidate the taxonomic status and morphological studies, 53 accessions of 12 Salvia species were collected from th...
Antimicrobial effect of aqueous and ethanolic extracts Teucrium polium L. on Streptococcus pyogenes, Pseudomonas aeruginosa and Staphylococcus epidermidis
Teucrium polium L. (Labiatae) has long been recognized in folk medicine in the treatment of many pathophysiological implications, such as gastrointestinal disorders, inflammations, diabetes and rheumatism. In this study...
Mechanisms of plant growth promoting rhizobacteria (PGPR) and mycorrhizae fungi to enhancement of plant growth under salinity stress: A review
Salinity is the major environmental factor limiting plant growth and productivity. Under salinity conditions, plant growth is affected by a number of factors such as hormonal and nutritional imbalance, ion toxicity, phys...
Study of genetic variation and phenetic relationships in some vulnerable taxa from Indian sundarbans following RAPD marker analysis
The mangrove ecosystem plays an important role in maintaining coastal ecological balance and is also one of the most highly productive and dynamic ecosystems to mankind, providing food, livelihood and ecological security...