L-Arginine optimization medium for hairy root induction of madder (Rubia tinctorum L.) based on one factor model of response surface methodology
Journal Title: Scientific Journal of Biological Sciences - Year 2014, Vol 3, Issue 12
Abstract
Medium optimization for hairy root cultures producing secondary metabolites was studied through statistical experimental design. In the following, one factor model of response surface methodology (RSM) was employed to formulate the L-Arginine amino acid levels alongside three categorical factors including bacterial strains (ATCC 15834, C58C1 and R1000), type of explant (Leaf and Stem) in co-cultivation media (B5 and MS) for hairy root induction of Madder (Rubia tinctorum L.). Design of experiment and data analysis was carried out by using Expert-Design® 7.1 software. According to the results, modeling and optimization conditions, including L-Arginine concentration 1.00 mM; bacterial strain; C58C1, leaf explant and B5 medium for HR induction frequency equal to 58% was evaluated (Desirability point=0.986). These optimal conditions predicted by RSM were confirmed to enhance hairy root induction as an application potential for biotechnological implementation to produce the anticipated compounds.
Authors and Affiliations
M. Ghorbani*| Master Degree of Agricultural Biotechnology, Agronomy and Plant Breeding Department, University of Tehran, IRAN., M. Omidi| Professor of Molecular Genetics and Biotechnology, Department of Agronomy and Plant Breeding, University of Tehran, IRAN., S. A. Peighambari| Professor in Plant Breeding, Department of Agronomy and Plant Breeding, University of Tehran, IRAN.
Keywords
Related Articles
- EP ID EP1114
- DOI 10.14196/sjbs.v3i12.1788
- Views 480
- Downloads 26
Sensibility of uropathogens in pregnant women with asymptomatic bacteriuria inLome, Togo
To investigate antibiotic therapy with pregnant women to asymptomatic bacteriuria (AB) in Prenatal Consultation (PNC) without prior urinary tract infection at the moment of the visit in Lome health center. The urines wer...
Histopathological study on the effects of oral administration of aqueous leaf extracts of cymbopogon citratus on the frontal cortex of male sprague dawley rats
This investigation was to evaluate the histopathological effects of oral consumption of the aqueous leaf extract of Cymbopogon citratus on the frontal cortex of Sprague Dawley (SD) rats. Ten male SD rats weighing between...
MED12 exon 2 mutations in uterine leiomyoma’s in women patients of Tehran Province of Iran
Uterine leiomyomas or fibroids are benign smooth muscle tumors of myometrial origin; frequently found in reproductive age women. Uterine leiomyomas commonly cause significant symptoms such as anemia resulting from heavy...
Haematologic and Biochemical Indices of Plasmodium falciparum Infected Inhabitants of Owerri, Imo State, Nigeria
The present study seeks to investigate changesin haematologic and biochemical indices of moderately P. falciparum infectedmale inhabitants of Owerri Municipality. Haematologic and biochemical indiceswere estimated by spe...
Recent progress in sustainable development and environmentally friendly energy systems: Case of Sudan
The move towards a low-carbon world, driven partly by climate science and partly by the business opportunities it offers, will need the promotion of environmentally friendly alternatives, if an acceptable stabilisation l...