Lead-induced oxidative stress and copper overload in neural stem cells in vitro and the protective effects of MitoQ
Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 3
Abstract
Objective To study the effects of lead ion (Pb2 + ) exposure on oxidative stress and copper overload in neural stem cells (NSC), and to explore the antioxidative protection of MitoQ. Methods C17. 2 neural stem cells were treated with 2. 5, 5, 10, and 20 μmol / L Pb(Ac)2 for 24 h and 48 h, respectively, to establish a low-dose Pb2 + exposure model in vitro, and the control group [0 μmol / L Pb(Ac)2] was set. Cell viability was detected by MTT assay. The content of reactive oxygen species ( ROS) was measured by DCFH-DA fluorescent probe, and the content of malondialdehyde (MDA) was determined by TBA method. The activity of superoxide dismutase 1 ( SOD1) was determined by xanthine oxidase method. Copper absorption was detected by atomic absorption spectrophotometry. The protein levels of CTR1, ATP7A and ATP7B were detected by Western blotting. Results Compared with the control group, 2. 5 - 20 μmol / L Pb2 + exposure for 24 h or 48 h significantly inhibited cell viability, while the levels of ROS and MDA and the activity of SOD1 were significantly higher than those of the control group ( P < 0. 01, P < 0. 05). After Pb2 + exposure, the intracellular copper level significantly increased ( P < 0. 05), CTR1 protein level increased ( P < 0. 05), while ATP7A and ATP7B protein level significantly decreased (P < 0. 01, P < 0. 05). Treatment with antioxidant MitoQ could reverse the changes of ROS, MDA and SOD1 induced by Pb2 + ( P < 0. 01 ), with a significant increase in cell viability. Conclusion Pb2 + exposure may promote oxidative stress and decrease cell viability by inducing copper overload in C17. 2 NSC. The antioxidant MitoQ can protect the cells from oxidative stress induced by Pb2 + exposure and promote the recovery of cell viability.
Authors and Affiliations
YAO Jinyu, WANG Tao, ZOU Yuankang, ZHAO Ziyu, GUAN Ruili, ZHENG Gang
Keywords
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