Maternal solubility test and high performance liquid chromatography of newborns in combination as a better neonatal screening protocol for sickle cell disease
Journal Title: International Journal of Medical Research and Review - Year 2016, Vol 4, Issue 6
Abstract
Introduction: The incidence and prevalence of sickle cell disease in India is high and they are major health problem in India. Neonatal detection and prophylactic management can reduce morbidity and mortality in childhood. A study is therefore planned for analyzing maternal solubility test and HPLC of newborn as effective screening protocol. Material and Method: The infants born of solubility positive mothers were taken for hemoglobin analysis using Biorad Hemoglobin variant by high performance liquid chromatography (HPLC) at birth and the test was repeated at six months of age. Data were analyzed using appropriate statistical method. Results: A total of 100 mothers were positive on solubility test, their infants underwent Hb analysis by high performance liquid chromatography. 74 infants shows normal hemoglobin variants while 19 were heterozygous for Hbs (sickle cell trait) and 7 babies were homozygous for HbS at birth. On follow up at 6 month of age cases were reanalyzed by HPLC, 12 cases were lost in follow up, 7 cases who were heterozygous for sickle cell turned out to be homozygous for sickle cell, and of those with normal reports 7 cases were homozygous. Conclusion: Maternal solubility test and HPLC of newborn at birth is good screening protocol for sickle cell anemia
Authors and Affiliations
Sharja Phuljhele, K. Ramnani, S. Hiwale, Priyanka Pruthviraj
Keywords
Related Articles
- EP ID EP230296
- DOI 10.17511
- Views 128
- Downloads 0
Comparison of conventional methods with gene xpert mtb/rif assay for rapid detection of mycobacterium tuberculosis and rifampicin resistance in extra-pulmonary samples
Introduction: Tuberculosis (TB) is a major health problem in India. The World Health Organization has recently in 2010 endorsed the Gene Xpert MTB/RIF assay for rapid detection of smear negative and multidrug resistance...
Tobacco use and mortality due to cardiovascular disease, malignancy and allcauses in a large cohort
Introduction: 67.6% of all Non-Communicable Disease (NCD) deaths are attributable to Cardiovascular Diseases (CVDs) and Cancers. Of the 14.2 million annual deaths attributable to modifiable behavioural factors, tobacco u...
Randomized control trial to compare the efficacy of split thickness transplant versus autologous melanocyte transplant in the management of stable vitiligo
Background: Stable cases of vitiligo can be managed surgically and over the years there has been a shift in the management protocol from the time tested epidermal tissue grafting techniques to the newer melanocyte or cel...
Transferrin levels in antenatal women
Introduction: During pregnancy, hemodilution leads to reduced hemoglobin, iron and ferritin concentration with increase in total iron binding capacity. Iron deficiency anemia is an important risk factor in pregnancy, att...
Laboratory surveillance of H1N1 swine influenza A virus infection in patients with severe acute respiratory illness (SARI)-an institutional study
Introduction: Lower respiratory tract infections (LRTI) are a leading cause of death in developing countries and are also third leading cause of death worldwide. Influenza is an acute viral and highly contagious respirat...