METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF DESVENLAFAXINE AND CLONAZEPAM IN BULK & TABLET FORMULATION BY RP-HPLC METHOD
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 4
Abstract
The present work is a simple, precise, accurate, efficient and reproducible RP-HPLC method was developed and validated for the simultaneous estimation of Desvenlafaxine and Clonazepam in combined dosage form. Chromatographic separation was effectively carried out on zodiac c18 (250mm X 4.6mm; 5μ) column and mobile phase contain KH2PO4 buffer, methanol and acetonitrile in a proportion of 30:20:50v/v at a flow rate of 1ml/min with U.V detection at 222nm.Retention times of Desvenlafaxine and Clonazepam was found to be 2.51 min and 4.40 min respectively. As per ICH guidelines the developed method was validated in terms of accuracy, Precission, linearity, LOD, LOQ, specificity and robustness. The linearity can occurs in between concentration range of 60-140mcg/ml and 0.6-1.4mcg/ml and the co relation co-efficients were 0.998 and 0.997 for Desvenlafaxine and Clonazepam respectively. The effectively developed and validated method can be used for estimation of Desvenlafaxine and clonazepam in tablet pharmaceutical dosage form.
Authors and Affiliations
Regalagadda Mallikarjuna, Nanda Kishore Agarwal, Prem Kumar Bichala, Sukhen som
Keywords
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