METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF DESVENLAFAXINE AND CLONAZEPAM IN BULK & TABLET FORMULATION BY RP-HPLC METHOD
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 4
Abstract
The present work is a simple, precise, accurate, efficient and reproducible RP-HPLC method was developed and validated for the simultaneous estimation of Desvenlafaxine and Clonazepam in combined dosage form. Chromatographic separation was effectively carried out on zodiac c18 (250mm X 4.6mm; 5μ) column and mobile phase contain KH2PO4 buffer, methanol and acetonitrile in a proportion of 30:20:50v/v at a flow rate of 1ml/min with U.V detection at 222nm.Retention times of Desvenlafaxine and Clonazepam was found to be 2.51 min and 4.40 min respectively. As per ICH guidelines the developed method was validated in terms of accuracy, Precission, linearity, LOD, LOQ, specificity and robustness. The linearity can occurs in between concentration range of 60-140mcg/ml and 0.6-1.4mcg/ml and the co relation co-efficients were 0.998 and 0.997 for Desvenlafaxine and Clonazepam respectively. The effectively developed and validated method can be used for estimation of Desvenlafaxine and clonazepam in tablet pharmaceutical dosage form.
Authors and Affiliations
Regalagadda Mallikarjuna, Nanda Kishore Agarwal, Prem Kumar Bichala, Sukhen som
Keywords
Related Articles
- EP ID EP33097
- DOI -
- Views 365
- Downloads 0
Bleaching of Echium amoenum Fisch & C.A.Mey by Gel anoinic, macroprus absorbing and macroprus anion resin for production of a drink by using date syrup
Date syrup is a suitable and natural sweetener for using in food stuffs formulation and improvement of its nutritional and sensory characteristics. In this research, to produce Echium amoenum Fisch & C.A.Mey drink with...
METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF DESVENLAFAXINE AND CLONAZEPAM IN BULK & TABLET FORMULATION BY RP-HPLC METHOD
The present work is a simple, precise, accurate, efficient and reproducible RP-HPLC method was developed and validated for the simultaneous estimation of Desvenlafaxine and Clonazepam in combined dosage form. Chromatog...
A review on uses of clove in oral and general health
Today, herbal medicine is becoming more and more popular as a safe and effective means of treatment for many different medical conditions. Herbs are often preferred because they are natural and do not put harmful chemi...
STABILITY INDICATING HPLC METHOD FOR THE ESTIMATION OF CINACALCET HYDROCHLORIDE API
A stability-indicating liquid chromatography method has been developed and validated for the determination of Cinacalcet hydrochloride in a laboratory mixture as well as in a tablet formulation developed in-house. Effi...
A study on biochemical parameters of diabetic nephropathy patients before and after the dialysis
Diabetes is the chronic metabolic disorder which disturb the metabolic pathways of protein, carbohydrate and lipid metabolism. It causes the microvascular complications like diabetic nephropathy. Diabetic neuropathy an...