METHOD DEVELOPMENT AND VALIDATION FOR THIRD GENERATION CEPHALOSPORIN BY UV-VIS SPECROPHOTOMETER 

Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2011, Vol 2, Issue 1

Abstract

The present study describes a simple, accurate, precise and cost effective UV-Vis Spectrophotometric method for the estimation of ceftazidime, a third generation cephalosporin anti‐biotic in dry powder injection and drug substances. The solvent used throughout the experiment was distilled water. The λmax or the absorption maxima of the drug was found at 275 nm. Beer’s law was obeyed in the range of 2.0-16.0 µg/ml. The developed method was successfully validated with respect to linearity, accuracy and precision. The sample concentrations are measured on weight basis throughout the experiment. The method was validated and shown linear in the mentioned concentrations. The correlation coefficient for ceftazidime was 0.9999. The recovery values for ceftazidime ranged from 99.9-100.3. The relative standard deviation of six replicates of assay was less than 2 %. The percent relative standard deviation of inter-day precision ranged 1.1-1.4 % and intra-day precision 1.2-1.6 % of ceftazidime. The limit of detection and limit of quantification of ceftazidime was 0.12 µg/ml and 0.46 µg/ml. The developed method was cross checked with high performance liquid chromatography for six replicate assays, the mean assay was 100.1% and %RSD was 0.35%.Hence proposed method was precise, accurate and cost effective. This method can be applicable for quantitative determination of the titled drug with respect to assay from their new commercial formulation of injection in quality control laboratories. 

Authors and Affiliations

A. B Devkhile , K. A Shaikh

Keywords

Related Articles

ANTIMICROBIAL ACTIVITY OF FEW SELECTED MEDICINAL PLANTS 

The petroleum ether, chloroform, methanol and aqueous extracts of leaves of Ageratum conyzoides Linn (Fam: Asteraceae), Argemone mexicana Linn. (Fam: Papaveraceae), Heliotropium indicum Linn (Fam: Boraginaceae) and stem...

METHANOL AND WATER EXTRACTS OF COSTUS SPECIOSUS (J.KÖNIG) SM. LEAVES REVERSE THE HIGH-FAT-DIET INDUCED PERIPHERAL INSULIN RESISTANCE IN EXPERIMENTAL WISTAR RATS  

 Worldwide prevalence of insulin resistance is growing rapidly and there is no specific treatment for this condition. Medicinal plants are being used as sources of novel drug discovery. The objective of this study w...

ANTI-INFLAMMATORY AND ANTIPYRETIC EFFECTS OF CARPOLOBIA LUTEA LEAF EXTRACT IN RODENTS 

The polar and non-polar fractions from the leaf of Carpolobia lutea G. Don (Family Polygalaceae) were assessed for anti-inflammatory and antipyretic effects. Anti-inflammatory activities were evaluated using acute anti-i...

BLOOD GLUCOSE CONTROL AND ITS DETERMINANTS 

Diabetes is a complex disorder that requires constant attention to diet, exercise, glucose monitoring, and medication to achieve good glycemic control. Diabetes affects up to 10 % of the population aged 20 years and olde...

COMPARATIVEANTIMICROBIAL ACTIVITIESOF NEEM AND CURRY LEAFEXTRACTS AND THEIR SYNERGISTIC EFFECT AGAINST SELECTED PATHOGENIC BACTERIA AND FUNGUS  

There is a growing demand today for naturally derived drugs and medicine in place of synthetic drugs that are more commercially available. Such drugs derived from indigenous plants are effective as they contain potent th...

Download PDF file
  • EP ID EP97703
  • DOI -
  • Views 143
  • Downloads 0

How To Cite

A. B Devkhile, K. A Shaikh (2011). METHOD DEVELOPMENT AND VALIDATION FOR THIRD GENERATION CEPHALOSPORIN BY UV-VIS SPECROPHOTOMETER . International Research Journal of Pharmacy (IRJP), 2(1), 222-229. https://europub.co.uk/articles/-A-97703