METHOD DEVELOPMENT AND VALIDATION FOR THIRD GENERATION CEPHALOSPORIN BY UV-VIS SPECROPHOTOMETER
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2011, Vol 2, Issue 1
Abstract
The present study describes a simple, accurate, precise and cost effective UV-Vis Spectrophotometric method for the estimation of ceftazidime, a third generation cephalosporin anti‐biotic in dry powder injection and drug substances. The solvent used throughout the experiment was distilled water. The λmax or the absorption maxima of the drug was found at 275 nm. Beer’s law was obeyed in the range of 2.0-16.0 µg/ml. The developed method was successfully validated with respect to linearity, accuracy and precision. The sample concentrations are measured on weight basis throughout the experiment. The method was validated and shown linear in the mentioned concentrations. The correlation coefficient for ceftazidime was 0.9999. The recovery values for ceftazidime ranged from 99.9-100.3. The relative standard deviation of six replicates of assay was less than 2 %. The percent relative standard deviation of inter-day precision ranged 1.1-1.4 % and intra-day precision 1.2-1.6 % of ceftazidime. The limit of detection and limit of quantification of ceftazidime was 0.12 µg/ml and 0.46 µg/ml. The developed method was cross checked with high performance liquid chromatography for six replicate assays, the mean assay was 100.1% and %RSD was 0.35%.Hence proposed method was precise, accurate and cost effective. This method can be applicable for quantitative determination of the titled drug with respect to assay from their new commercial formulation of injection in quality control laboratories.
Authors and Affiliations
A. B Devkhile , K. A Shaikh
Keywords
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- EP ID EP97703
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