METHOD DEVELOPMENT AND VALIDATION OF METFORMIN, GLIMEPIRIDE AND PIOGLITAZONE IN TABLET DOSAGE FORM BY RP-HPLC
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 8
Abstract
A rapid RP-HPLC method was developed and validated for simultaneous estimation of metformin, glimepiride and pioglitazone from pharmaceutical dosage forms. A sensitive chromatographic separation was accomplished on C18 column (100 × 4.6 mm, 5 µ) with mobile phase consisting of Methanol: phosphate buffer (pH 3.6 adjusted with ortho phosphoric acid) in the ratio of 75:25 v/v, at a flow rate of 1.0 ml / min and monitored at 238 nm. The developed method was validated in terms of accuracy, precision, linearity and limit of detection, limit of quantification, robustness and solution stability. The proposed method can be used for the routine estimation of these drugs in combined pharmaceutical dosage forms.
Authors and Affiliations
Suchitra M , Sunitha D , Parthiban C , Siddartha B , Madhavi C
Keywords
Related Articles
- EP ID EP104264
- DOI 10.7897/2230-8407.04850
- Views 117
- Downloads 0
EXPERIMENTAL STUDIES ON ACTIVE METABOLITES OF CALOTROPIS GIGANTEA FOR EVALUATION OF POSSIBLE ANTIFUNGAL PROPERTIES
The active metabolites like phytochemicals from the medicinal plants were under exploration for the development of novel and biodegradable effective drugs as an alternative to the ineffective contemporary medicine. In th...
DEVELOPMENT AND VALIDATION OF A STABILITY INDICATING RP-HPLC METHOD FOR DETERMINATION OF ESLICARBAZEPINE IN ESLICARBAZEPINE ACETATE TABLETS
A simple, precise, rapid and accurate stability indicating reverse phase high performance liquid chromatography has been developed and validated for the estimation of Eslicarbazepine Acetate in tablet dosage form. Separa...
A TWELVE-MONTHLY REVIEW ON ANTI-DIABETIC PLANTS: JAN-DEC-2011
One of the commonest endocrine disorders is diabetes mellitus, that is characterized by insufficient or lack of insulin and impaired carbohydrate metabolism in the body leading to excretion of sweet urine and long-term c...
PHYTOCHEMICAL SCREENING OF SECONDARY METABOLITES FROM MYXOPYRUM SERRATULUM A.W. HILLS BY GC-MS, HPTLC ANALYSIS
Myxopyrum serratulum is a valuable medicinal plant widely distributed in Western Ghats at the altitude of 600-900m. Traditionally, the leaves of the plant used to treat asthma, cough diabetes and nerve complaints. The cu...
FORMULATION AND EVALUATION OF SUSTAINED-RELEASE FLOATING TABLETS OF CIPROFLOXACIN
The aim of this study was to develop a sustained-release floating system that is able to float over an extended period of time. Ciprofloxacin was used as a model drug. The system consisted of a 3 mm drug-containing gas-g...